|Tested species reactivity||Virus|
|Host / Isotype||Guinea pig / IgG|
|Immunogen||Recombinant p17 protein|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4956 detects the HIV-1 p17 protein in HIV samples.
PA1-4956 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~17 kDa protein, and a ~55 kDa protein, corresponding respectively to HIV-p17 and to its precursor p55, in HIV samples.
PA1-4956 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the Gag p17 coding region of the HIV-1 virus.
Recombinant HIV-1 Gag p17 protein (Cat. # RP-4914 or RP-4915) can be purchased for control experiments in Western blot assays.
HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the of AIDS virus particle construction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.