|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified membrane of human tonsil cells.|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/1 million cells|
|Functional Assay (FN)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70111 detects HLA-ABC in human samples.
MA1-70111 has been successfully used in flow cytometry, functional assay, and immunoprecipitation procedures.
The MA1-70111 immunogen is purified membrane of human tonsil cells.
Store at 4°C short term. For extended storage aliquot and store at -20°C. Avoid Freeze/Thaw Cycles.
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis C virus infection in inclusion body myositis: A case-control study.
MA1-70111 was used in immunohistochemistry - paraffin section to determine if there is an association between inclusion body myositis and hepatitis C virus infection
|Uruha A,Noguchi S,Hayashi YK,Tsuburaya RS,Yonekawa T,Nonaka I,Nishino I||Neurology (86:211)||2016|
Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.
MA1-70111 was used in immunocytochemistry to study the role of clathrin-dependent and clathrin-independent routes in the uptake of Helicobacter pylori vesicles by gastric epithelial cells
|Olofsson A,Nygård Skalman L,Obi I,Lundmark R,Arnqvist A||mBio (5:e00979)||2014|
Alterations in the Arf6-regulated plasma membrane endosomal recycling pathway in cells overexpressing the tetraspan protein Gas3/PMP22.
MA1-70111 was used in immunocytochemistry to study alterations in Arf6-regulated plasma membrane endosomal recycling in cells overexpressing Gas3/PMP22
|Chies R,Nobbio L,Edomi P,Schenone A,Schneider C,Brancolini C||Journal of cell science (116:987)||2003|
Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.
MA1-70111 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells
|Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK||Anticancer research (33:4875)||2013|
Induction of metastatic cancer stem cells from the NK/LAK-resistant floating, but not adherent, subset of the UP-LN1 carcinoma cell line by IFN-¿.
MA1-70111 was used in flow cytometry to study the induction of metastatic cancer stem cells from UP-LN1 cell line
|Chen HC,Chou AS,Liu YC,Hsieh CH,Kang CC,Pang ST,Yeh CT,Liu HP,Liao SK||Laboratory investigation; a journal of technical methods and pathology (91:1502)||2011|
|Not Applicable||Not Cited||
The human herpesvirus-7 (HHV-7) U21 immunoevasin subverts NK-mediated cytoxicity through modulation of MICA and MICB.
MA1-70111 was used in western blot to report that the human herpesvirus-7 U21 gene product interferes with natural killer cell recognition
|Schneider CL,Hudson AW||PLoS pathogens (7:null)||2011|
Establishment of a quantitative ELISA capable of determining peptide - MHC class I interaction.
MA1-70111 was used in ELISA to develop a quantitative ELISA assay for studying peptide-MHC class I interactions
|Sylvester-Hvid C,Kristensen N,Blicher T,Ferré H,Lauemøller SL,Wolf XA,Lamberth K,Nissen MH,Pedersen LØ,Buus S||Tissue antigens (59:251)||2002|