OriGene

HOXC4 Monoclonal Antibody (OTI2D5), TrueMAB™

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HOXC4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-HOXC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809708)(1:2000).

HOXC4 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC4 (RC223682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC4. Positive lysates LY406994 (100 µg) and LC406994 (20 µg) can be purchased separately from OriGene.

Product Details

CF809708

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	OTI2D5
Immunogen	Full length human recombinant protein of human HOXC4 produced in E.coli. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homeo box 3E; homeo box C4; Homeobox protein CP19; Homeobox protein Hox-3.5; Homeobox protein Hox-3E; Homeobox protein Hox-C4; Hox-35

Gene Aliases: cp19; Hox-3.5; HOX3; HOX3E; Hoxc-4; HOXC4

UniProt ID: (Human) P09017, (Mouse) Q08624

Entrez Gene ID: (Human) 3221, (Mouse) 15423

Function(s)

skeletal system development transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development anterior/posterior pattern specification embryonic organ morphogenesis cartilage development

Process(es)

transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding HMG box domain binding DNA binding