OriGene

HSD17B10 Monoclonal Antibody (OTI5E2), TrueMAB™

Product Image Gallery

HSD17B10 Antibody in Immunofluorescence (IF) — Anti-HSD17B10 mouse monoclonal antibody (TA500813) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B10(RC201734).

HSD17B10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSD17B10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500813, Dilution 1:50)

HSD17B10 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B10 (RC201734, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD. Positive lysates LY401426 (100 µg) and LC401426 (20 µg) can be purchased separately from OriGene.

HSD17B10 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Hela cells, using anti-HSD17B10 antibody (TA500813), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Western Blot (WB)

1:2000

Product Specifications

Species

Human

Expression System

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI5E2

Immunogen

Full length human recombinant protein of human HSD17B10 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 17-beta-HSD 10; 17-beta-hydroxysteroid dehydrogenase 10; 2-methyl-3-hydroxybutyryl-CoA dehydrogenase; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; 3-hydroxyacyl-CoA dehydrogenase type-2; AB-binding alcohol dehydrogenase; amyloid-beta peptide binding alcohol dehydrogenase; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; HADH2 protein; hydroxysteroid (17-beta) dehydrogenase 10; hydroxysteroid dehydrogenase 10; MHBD; mitochondrial ribo; Mitochondrial ribonuclease P protein 2; mitochondrial RNas; Mitochondrial RNase P protein 2; mitochondrial RNase P subunit 2; RP3-339A18.2; Short chain dehydrogenase/reductase family 5C member 1; short chain dehydrogenase/reductase family 5C, member 1; short chain L-3-hydroxyacyl-CoA dehydrogenase type 2; short chain type dehydrogenase/reductase XH98G2; Short-chain type dehydrogenase/reductase XH98G2; type 10 17b-HSD; Type II HADH

Gene Aliases: 17b-HSD10; ABAD; CAMR; DUPXp11.22; ERAB; HADH2; HCD2; HSD17B10; MHBD; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1; XH98G2

UniProt ID: (Human) Q99714

Entrez Gene ID: (Human) 3028

Molecular Function: dehydrogenase oxidoreductase reductase