|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11213 targets Heat Shock Protein 27 in IHC (P) and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-11213 immunogen is partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Hsp27 is identical to the estrogen-induced "p29" and "24K" protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Multiplexed high content screening reveals that cigarette smoke condensate-altered cell signaling pathways are accentuated through FAK inhibition in human bronchial cells.
MA5-11213 was used in immunocytochemistry to study the role of FAK in the mechanism by which cigarette smoke condensate exerts its effects on human bronchial cells
|Carter CA||International journal of toxicology (31:257)||2012|
Altered expression of HSP27 and HSP70 in distal oesophageal mucosa in patients with gastro-oesophageal reflux disease subjected to fundoplication.
MA5-11213 was used in immunohistochemistry to investigate the effect of heat shock protein 27 and heat shock protein 70 on mucosal defence system of the distal oesophagus
|Rantanen T,Honkanen T,Paavonen T,Rantanen L,Oksala N||European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology (37:168)||2011|
[The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis].
MA5-11213 was used in immunohistochemistry to examine the prognostic significance of multiple genes in osteosarcoma
|Ozger H,Eralp L,Atalar AC,Toker B,Esberk Ate¿ L,Sungur M,Bilgiç B,Ayan I||Acta orthopaedica et traumatologica turcica (43:28)||2009|
Expressions of HSP70 and HSP27 in hepatocellular carcinoma.
MA5-11213 was used in immunohistochemistry to study the expressions of heat shock proteins in hepatocellular carcinoma and their role in carcinogenesis
|Joo M,Chi JG,Lee H||Journal of Korean medical science (20:829)||2005|
Immunohistochemical study of heat shock proteins 27, 60 and 70 in the normal human adrenal and in adrenal tumors with suppressed ACTH production.
MA5-11213 was used in immunohistochemistry to investigate the expression of heat shock proteins in normal and tumoral adrenal glands
|Pignatelli D,Ferreira J,Soares P,Costa MJ,Magalhães MC||Microscopy research and technique (61:315)||2003|
Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries.
MA5-11213 was used in immunohistochemistry to study the expression of heat-shock proteins in malignant epithelial ovarian tumors
|Elpek GO,Karaveli S,Sim¿ek T,Keles N,Aksoy NH||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (111:523)||2003|
Polyglutamine expansions cause decreased CRE-mediated transcription and early gene expression changes prior to cell death in an inducible cell model of Huntington's disease.
MA5-11213 was used in immunohistochemistry to investigate the effect of polyglutamine expansions on gene expression changes in a Huntington disease model
|Wyttenbach A,Swartz J,Kita H,Thykjaer T,Carmichael J,Bradley J,Brown R,Maxwell M,Schapira A,Orntoft TF,Kato K,Rubinsztein DC||Human molecular genetics (10:1829)||2001|
Differential expression of Hsp27 in normal oesophagus, Barrett's metaplasia and oesophageal adenocarcinomas.
MA5-11213 was used in immunohistochemistry to investigate the expression of heat shock protein 27 in normal and malignant cells
|Soldes OS,Kuick RD,Thompson IA,Hughes SJ,Orringer MB,Iannettoni MD,Hanash SM,Beer DG||British journal of cancer (79:595)||1999|
Heat shock proteins hsp27 and hsp70: lack of correlation with response to tamoxifen and clinical course of disease in estrogen receptor-positive metastatic breast cancer (a Southwest Oncology Group Study).
MA5-11213 was used in immunohistochemistry to study whether hsp27 and hsp70 expression is correlated with clinical resistance to tamoxifen in ER-positive metastatic breast cancer
|Ciocca DR,Green S,Elledge RM,Clark GM,Pugh R,Ravdin P,Lew D,Martino S,Osborne CK||Clinical cancer research : an official journal of the American Association for Cancer Research (4:1263)||1998|
Expression of heat shock proteins in squamous cell carcinoma of the tongue: an immunohistochemical study.
MA5-11213 was used in immunohistochemistry to investigate the distribution of heat shock protein proteins in squamous cell carcinoma of the tongue
|Ito T,Kawabe R,Kurasono Y,Hara M,Kitamura H,Fujita K,Kanisawa M||Journal of oral pathology and medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (27:18)||1998|
Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.
MA5-11213 was used in western blot to study the role of 14-3-3sigma in drug resistance in human breast cancer cells
|Liu Y,Liu H,Han B,Zhang JT||Cancer research (66:3248)||2006|
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
MA5-11213 was used in western blot to study the role of the c-Yes 3'-UTR in mRNA decay in breast cancer cells
|Sommer S,Cui Y,Brewer G,Fuqua SA||The Journal of steroid biochemistry and molecular biology (97:219)||2005|
Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells.
MA5-11213 was used in western blot to study the induction of apoptosis and inactivation of the multi-chaperone complex in human epidermal cancer cells by electromagnetic fields at mobile phone frequency
|Caraglia M,Marra M,Mancinelli F,D'Ambrosio G,Massa R,Giordano A,Budillon A,Abbruzzese A,Bismuto E||Journal of cellular physiology (204:539)||2005|
Differential proteome analysis of replicative senescence in rat embryo fibroblasts.
MA5-11213 was used in western blot to perform a deifferential proteomic analysis of replicative senescence in rat embryo fibroblasts
|Benvenuti S,Cramer R,Quinn CC,Bruce J,Zvelebil M,Corless S,Bond J,Yang A,Hockfield S,Burlingame AL,Waterfield MD,Jat PS||Molecular and cellular proteomics : MCP (1:280)||2002|
The Fas-induced apoptosis analyzed by high throughput proteome analysis.
MA5-11213 was used in western blot to perform a proteomic analysis of Jurkat T-cell cytosolic proteins during Fas-induced cell death
|Gerner C,Frohwein U,Gotzmann J,Bayer E,Gelbmann D,Bursch W,Schulte-Hermann R||The Journal of biological chemistry (275:39018)||2000|