Immunofluorescent analysis of Heat Shock Protein 90 beta (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Heat Shock Protein 90 beta polyclonal antibody (Product # PA5-16327) at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Pig, Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to aa 2-13 (PEEVHHGEEEVE-Cys) from the N-terminus end of mouse heat shock protein 90beta|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug/ml|
|Western Blot (WB)||1-10 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16327 targets Heat Shock Protein 90 beta in IP, IF/ICC, and WB applications and shows reactivity with mouse, Rat, and Human samples.
The PA5-16327 immunogen is a synthetic peptiude corresponding to aa 2-13 (PEEVHHGEEEVE-Cys) from the N-terminus end of mouse heat shock protein 90beta.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Cells respond to heat and chemical stress by inducing the synthesis of a group of proteins referred to as heat shock proteins. HSP90 is present in cells at high levels in the cytoplasm and is composed of two separate gene products, HSP90alpha and HSP90beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential proteome and transcriptome analysis of porcine skeletal muscle during development.
PA5-16327 was used in western blot to study developmental changes in pig skeletal muscle at the transcriptomic and proteomic levels
|Xu Y,Qian H,Feng X,Xiong Y,Lei M,Ren Z,Zuo B,Xu D,Ma Y,Yuan H||Journal of proteomics (75:2093)||2012|
|Not Applicable||Not Cited||
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
PA5-16327 was used in immunocytochemistry, immunoprecipitation, and western blot to elucidate the molecular cause of the abnormal behavior of Hectd1 mutant cranial mesenchyme
|Sarkar AA,Zohn IE||The Journal of cell biology (196:789)||2012|
Functional complexity of the axonal growth cone: a proteomic analysis.
PA5-16327 was used in immunohistochemistry to study the proteomics of the axonal growth cone
|Estrada-Bernal A,Sanford SD,Sosa LJ,Simon GC,Hansen KC,Pfenninger KH||PloS one (7:null)||2012|