|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human HSP70 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-15578 targets HSP70 in IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15578 immunogen is purified recombinant fragment of human HSP70 expressed in E. Coli.
MA5-15578 detects HSP70 which has a predicted molecular weight of approximately 70kDa.
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Heteromeric complexes of heat shock protein 70 (HSP70) family members, including Hsp70B', in differentiated human neuronal cells.
MA5-15578 was used in western blot to study heteromeric complexes of heat shock protein 70 family members in differentiated human neuronal cells
|Chow AM,Mok P,Xiao D,Khalouei S,Brown IR||Cell stress and chaperones (15:545)||2010|