|Tested species reactivity||Virus|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||HB-Xag-Protein A Fusion protein.|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
A suggested positive control for immunohistochemical applications is liver carcinoma/hepatitis B infected liver. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries. Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Hepatitis B virus X protein enhances Myc stability by inhibiting SCF(Skp2) ubiquitin E3 ligase-mediated Myc ubiquitination and contributes to oncogenesis.
MA1-81021 was used in immunocytochemistry to explore the mechanism by which HBx inhibits Myc ubiquitination.
|Lee S,Kim W,Ko C,Ryu WS||Oncogene (35:1857)||2016|