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Western blot analysis using Hepatitis C Virus NS5a Antigen Monoclonal Antibody (MA1-7368) was performed by loading 10µg or 25µg of whole cell extracts from human hepatoma cells (Huh7) or from Huh7 cells expressing a sub-genomic replicon of a hepatitis C virus (Huh7-Replicon). Membranes were probed with MA1-7368 (1:500), followed by a HRP-conjugated secondary antibody and chemiluminescent substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Virus|
|Published species reactivity||Virus|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:20 - 1:200|
|Immunofluorescence (IF)||1:10 - 1:50|
|Western Blot (WB)||1:10 - 1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
The MA1-7368 antibody reacts with Hepatitis C virus NS5a in viral and infected tissue samples. Positive for genotypes 1a and 1b, does not react with genotype 2a.
MA1-7368 has been successfully used in ELISA, Immunofluorescence, and Western blot applications.
The MA1-7368 antibody was raised against HCV NS5a protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ethanol facilitates hepatitis C virus replication via up-regulation of GW182 and heat shock protein 90 in human hepatoma cells.
MA1-7368 was used in immunocytochemistry to study the role of GW182 and Hsp90 in the ethanol-induced upregulation of HCV replication in human hepatoma cells
|Bukong TN,Hou W,Kodys K,Szabo G||Hepatology (Baltimore, Md.) (57:70)||2013|