Immunofluorescent analysis of Histone H2A.Z+H2A.F.Z in paraformaldehyde-fixed A431 cells using a Histone H2A.Z+H2A.F.Z polyclonal antibody (Product # PA5-21928) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 45 of Histone H2AZ|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21928 targets Histone H2A.Z/H2A.F/Z in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-21928 immunogen is a synthetic peptide corresponding to a region within amino acids 1 and 45 of Histone H2AZ.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.