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Histone extracts of HeLa cells were analyzed by western blot using the anti-H3K79me1 crude serum (Cat. no. 49-1018), diluted 1:1,000 and 1:2,000 in TBS-Tween containing 5% milk. The molecular weight marker is shown on the left. The location of the protein of interest is indicated with an arrow.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||rabbits against histone H3 containing the monomethylated lysine 79 [K79me1], using a KLH-conjugated synthetic peptide|
|Storage buffer||whole serum|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5-10 ug|
|ELISA (ELISA)||1:500 to 1:1,000|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post tranlationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
H3 histone family, member A, histone 1, H3a, histone H3/a, H3K79me1, Histone 1, H3a, HIST1H3A