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|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||human leukocyte interferon|
|Purification||Ion-exchange chromatography, Size-exclusion chromatography|
|Storage buffer||0.05M citric acid/PBS, pH 7.0|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Specificity: Neutralizes human interferon alpha; binds to human IFN alpha with high affinity (Ka=10^9 M^-1).
Activity: Interferon was titrated with the use of the cytopathic effect inhibition assay, where 1 unit/ml of interferon was necessary to produce an endpoint of 50%. One neutralization unit is defined as the amount of serum required to neutralize one unit of human IFN alpha to an endpoint of 50%.
Purity is >95%. Endotoxin level <1 EU/ug.
Store at -20°C for short-term use (less than 6 months). For long-term storage, aliquot and store at -70°C or below for retention of full activity. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% BSA.
Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma; MIM 147570) is produced by mitogen- or antigen-stimulated T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Type I interferons are involved in the development of ultraviolet B-induced inflammatory skin lesions in systemic lupus erythaematosus patients.
211002 was used in immunohistochemistry - paraffin section to study the involvement of type I interferons and endothelial cell adhesion molecules in the development of ultraviolet B-induced systemic lupus erythaematosus skin lesions
|Reefman E,Kuiper H,Limburg PC,Kallenberg CG,Bijl M||Annals of the rheumatic diseases (67:11)||2008|
|Not Applicable||Not Cited||
Stimulated plasmacytoid dendritic cells impair human T-cell development.
211002 was used in flow cytometry to report that IFN-alpha inhibits the IL-7R signal transduction pathway
|Schmidlin H,Dontje W,Groot F,Ligthart SJ,Colantonio AD,Oud ME,Schilder-Tol EJ,Spaargaren M,Spits H,Uittenbogaart CH,Blom B||Blood (108:3792)||2006|
IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; Interferon alpha-1/13; Interferon alpha-D; interferon, alpha 1; interferon-alpha1; LeIF D
IFL; IFN; IFN-ALPHA; IFN-alphaD; IFNA1; IFNA13; IFNA@