Mouse thiogylcolate-induced peritoneal exudate cells (PECs) were stimulated with LPS in the presence of Brefeldin A for 22 hours. The cells were surface stained with Anti-Mouse CD11b PE and then intracellularly stained with Rat IgG2b kappa Isotype Control FITC (top) or Anti-Mouse IL-1 alpha FITC (bottom, A18463).
|Tested species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||mouse interleukin-1 alpha|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
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