Invitrogen

IL-1 beta Recombinant Polyclonal Antibody (1HCLC)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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IL-1 beta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Interleukin-1 beta was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IL-1 beta Recombinant Polyclonal Antibody (1HCLC) (Product # 710270) at 5µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731, 1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

IL-1 beta Antibody in Western Blot (WB) — Western blot was performed using Anti-IL-1 beta Recombinant Polyclonal Antibody (1HCLC) (Product # 710270) and a 26 kDa band corresponding to Interleukin-1 beta was observed across all the tested cell lines and tissues, except Mouse Ovary, and was increased upon Lipopolysaccharide treatment. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 (Lipopolysaccharide (1 µg/mL), 18 hours) (Lane 2), RAW 264.7 (Lane 3), Mouse Spleen (Lane 4), Mouse Ovary (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). The 26 kDa form represents an intermediate fragment, which is produced by Metallo-protease mediated cleavage.

IL-1 beta Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using IL-1 beta Recombinant Polyclonal Antibody (1HCLC) (Product # 710270), shows an increase in expression in THP-1 upon treatment with Lipopolysaccharide. {TM}

Product Details

710270

Applications	Tested Dilution	Publications
Western Blot (WB)	1-10 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Recombinant Polyclonal
Type	Antibody
Clone	1HCLC
Immunogen	Recombinant protein corresponding to amino acids 118–269 of mouse IL-1b View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2532657

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta

Gene Aliases: IL-1; Il-1b; IL-1beta; IL1-BETA; IL1B; IL1F2

UniProt ID: (Human) P01584, (Mouse) P10749

Entrez Gene ID: (Human) 3553, (Mouse) 16176

Molecular Function: interleukin superfamily