|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-12|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM121B targets IL-12 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM121B immunogen is recombinant mouse IL-12.
MM121B detects IL-12 which has a predicted molecular weight of approximately 22 kDa.
The MM121B mouse IL12 antibody (biotinylated conjugate of clone C17-8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM120 (clone 9A5). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody MM121B (clone C17-8) and antibody MM120 (clone 9A5) have successfully been used in combination with recombinant mouse IL12p70 protein SMIL12 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A polymorphism in the coding region of Il12b promotes IL-12p70 and IL-23 heterodimer formation.
MM121B was used in western blot to investigate the effect of a polymorphism in the coding region of Il12b for interleukin-12p70 and interleukin-23 heterodimer formation
|Zwiers A,Fuss IJ,Seegers D,Konijn T,Garcia-Vallejo JJ,Samsom JN,Strober W,Kraal G,Bouma G||Journal of immunology (Baltimore, Md. : 1950) (186:3572)||2011|
Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes.
MM121B was used in ELISA to investigate the role of listeriolysin O in the calpain activation and interleukin-1 alpha secretion in Listeria monocytogene-infected macrophages
|Dewamitta SR,Nomura T,Kawamura I,Hara H,Tsuchiya K,Kurenuma T,Shen Y,Daim S,Yamamoto T,Qu H,Sakai S,Xu Y,Mitsuyama M||Infection and immunity (78:1884)||2010|
1-Methylnicotinamide and nicotinamide: two related anti-inflammatory agents that differentially affect the functions of activated macrophages.
MM121B was used in ELISA to study the effects of 1-methylnicotinamide and nicotinamide on the generation of inflammatory mediators by macrophages
|Biedro¿ R,Ciszek M,Tokarczyk M,Bobek M,Kurnyta M,S¿ominska EM,Smole¿ski RT,Marcinkiewicz J||Archivum immunologiae et therapiae experimentalis (56:127)||2008|
Heligmosomoides polygyrus promotes regulatory T-cell cytokine production in the murine normal distal intestine.
MM121B was used in ELISA to investigate the effect of exposure to Heligmosomoides polygyrus on cytokine pathways in murine normal distal intestine
|Setiawan T,Metwali A,Blum AM,Ince MN,Urban JF,Elliott DE,Weinstock JV||Infection and immunity (75:4655)||2007|
The influence of collagenase treatment on the production of TNF-alpha, IL-6 and IL-10 by testicular macrophages.
MM121B was used in ELISA to study the effect of collagenase treatment on cytokine production by testicular macrophages
|Bryniarski K,Szczepanik M,Ptak M,Ptak W||Journal of immunological methods (301:186)||2005|
Modulation of testicular macrophage activity by collagenase.
MM121B was used in ELISA to investigate the regulation of cytokines and the expression of ROI in testicular macrophages
|Bryniarski K,Szczepanik M,Ptak M,Ptak W||Folia histochemica et cytobiologica (43:37)||2005|
Heligmosomoides polygyrus inhibits established colitis in IL-10-deficient mice.
MM121B was used in ELISA to investigate the effect of Heligmosomoides polygyrus on colitis
|Elliott DE,Setiawan T,Metwali A,Blum A,Urban JF,Weinstock JV||European journal of immunology (34:2690)||2004|
Quantities of interleukin-12p40 in mature CD8alpha negative dendritic cells correlate with strength of TCR signal and determine Th cell development.
MM121B was used in ELISA and flow cytometry to report that the strength of TCR signaling directs Th cell development by regulating CD40L expression on T cells which determines production of IL-12p40 from DC via CD40.
|Fujimoto N,Ishida H,Nakamura I,Ogasawara K,Itoh Y||Microbiology and immunology (47:1017)||2003|
Modulation of macrophage activity by proteolytic enzymes. Differential regulation of IL-6 and reactive oxygen intermediates (ROIs) synthesis as a possible homeostatic mechanism in the control of inflammation.
MM121B was used in ELISA to investigate the effect of collagenase and trypsin for the regulation of macrophage function
|Bryniarski K,Maresz K,Szczepanik M,Ptak M,Ptak W||Inflammation (27:333)||2003|
NF-kappa B is required within the innate immune system to inhibit microflora-induced colitis and expression of IL-12 p40.
MM121B was used in ELISA to study the role of NF-kappa B and the innate immune system in inhibiting microflora-induced colitis
|Tomczak MF,Erdman SE,Poutahidis T,Rogers AB,Holcombe H,Plank B,Fox JG,Horwitz BH||Journal of immunology (Baltimore, Md. : 1950) (171:1484)||2003|
Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni.
MM121B was used in ELISA to investigate the promoting effect of interleukin 12 and schistosome egg antigen on substance P receptor expression
|Blum AM,Metwali A,Crawford C,Li J,Qadir K,Elliott DE,Weinstock JV||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (15:950)||2001|
Differential stimulation requirements for IL-12 production among clones of macrophage hybridomas.
MM121B was used in ELISA to investigate the effect of interferon gamma and LPS on interleukin 12 production from macrophage hybridomas
|Taneichi M,Horino A,Naito S,Sakurai S,Komuro K,Uchida T||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (17:603)||1997|
In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice.
MM121B was used in blocking or activating experiment to characterize specific memory T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice
|Kirby AC,Sundquist M,Wick MJ||Infection and immunity (72:6390)||2004|
Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination.
MM121B was used in blocking/activating experiment to investigate the effect of dendritic cell vaccination for the prevention of tumor migration
|Kim A,Noh YW,Kim KD,Jang YS,Choe YK,Lim JS||Experimental and molecular medicine (36:428)||2004|
CD40 ligation prevents neonatal induction of transplantation tolerance.
MM121B was used in blocking/activating experiment to study the effect of CD40 ligation on neonatal transplantation tolerance
|Flamand V,Donckier V,Demoor FX,Le Moine A,Matthys P,Vanderhaeghen ML,Tagawa Y,Iwakura Y,Billiau A,Abramowicz D,Goldman M||Journal of immunology (Baltimore, Md. : 1950) (160:4666)||1998|