Invitrogen

IL-13 Monoclonal Antibody (JES10-5A2)

Product Image Gallery

IL-13 Antibody in Flow Cytometry (Flow) — Human IL-13 Antibody. Isolated human PBMCs were stimulated and stained with the rat anti-human IL-13 R-PE. Data obtained represent the gated lymphocyte population. The diagram shows stimulated lymphocytes stained with rat anti-human IL-13 R-PE.

IL-13 Antibody in Western Blot (WB) — Published figure using IL-13 monoclonal antibody (Product # AHC0132) in Western Blot

IL-13 Antibody in Neutralization (Neu) — Fig. 4 The effect of IL-13 neutralization on RUNX2 expression in C2C12 cells. (a-c) Blocking IL - 13 activity with a neutralizing antibody (0.5 mug/ml) reduced RUNX2 protein level in C2C12 cells treated with tissue extracts from eosinophilic CRSwNP. Whole cell extracts were immunoblotted with the indicated antibodies. (d) Osteoblast differentiation was monitored by staining ALP and by measuring its activity at day 7. Calcium deposition was assessed by staining with alizarin red S at day 21. Data are expressed as mean +- SD. * P < .05, ** P < .01. Scale bar = 200 mum. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Fig. 4

Product Details

AHC0132

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 1 publication 1 publication
Immunohistochemistry (IHC)	Assay-dependent	-
Flow Cytometry (Flow)	Assay-dependent	View 1 publication 1 publication
ELISA (ELISA)	Assay-dependent	View 1 publication 1 publication
Neutralization (Neu)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rat / IgG1
Class	Monoclonal
Type	Antibody
Clone	JES10-5A2
Immunogen	Purified recombinant human IL-13
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	purified
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20°C
RRID	AB_2536246

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer.

Cancer immunology research

Kvorjak M,Ahmed Y,Miller ML,Sriram R,Coronnello C,Hashash JG,Hartman DJ,Telmer CA,Miskov-Zivanov N,Finn OJ,Cascio S

AHC0132 was used in an ELISA assay to investigate whether glycosylation of MUC1 to form MUC1 oncoproteins is influenced by macrophage-associated cytokines.

Sat Feb 01 00:00:00 EST 2020

Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer.

Cancer immunology research

Kvorjak M,Ahmed Y,Miller ML,Sriram R,Coronnello C,Hashash JG,Hartman DJ,Telmer CA,Miskov-Zivanov N,Finn OJ,Cascio S

AHC0132 was used in an ELISA assay to investigate whether glycosylation of MUC1 to form MUC1 oncoproteins is influenced by macrophage-associated cytokines.

Sat Feb 01 00:00:00 EST 2020

Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2.

EBioMedicine

Khalmuratova R,Shin HW,Kim DW,Park JW

Published figure using IL-13 monoclonal antibody (Product # AHC0132) in Neutralization

Thu Aug 01 00:00:00 EDT 2019

Variation of T-cell activation in allergic subjects during natural pollen exposure.

Allergy

Koscher V,Milhe F,El Biaze M,Vervloet D,Magnan A

AHC0132 was used in flow cytometry to compare the Th1/Th2 balance in cypress allergic patients with controls

Sun Jan 01 00:00:00 EST 2006

Assessment of T lymphocyte cytokine production in induced sputum from asthmatics: a flow cytometry study.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Boniface S,Koscher V,Mamessier E,El Biaze M,Dupuy P,Lorec AM,Guillot C,Badier M,Bongrand P,Vervloet D,Magnan A

AHC0132 was used in immunocytochemistry to measure T cell cytokine production in induced sputum.

Mon Sep 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody

Gene Aliases: IL-13; IL13; NC30; P600

UniProt ID: (Human) P35225

Entrez Gene ID: (Human) 3596

Function(s)