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Sandwich ELISA analysis of mouse IL-2 was performed using the a Mouse IL-2 Colorimetric ELISA Kit (Product # EMIL2) by loading 50 ul per well of an anti-mouse IL-2 monoclonal antibody (Product # MM100) in quadruplicate at 0 (blue line), 50% (2.5 ug/ml, red line), and 100% (5 ug/ml, green line) displacement in a plate and then loading 50 ul per well of Mouse IL-2 Recombinant Protein (Product # SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/ml across a 5 ug/ml Mouse IL-2 (Product # MM101) pre-coated plate and incubating for 2 hours at 37°C. The plate was washed, then incubated with 100 ul per well of an HRP-conjugated rat anti-mouse IL-2 antibody at a 1:25,000 dilution for 1 hour at 37°C. Detection was performed using TMB substrate solution for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Recombinant mouse IL-2|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM101 targets IL-2 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM101 immunogen is recombinant mouse IL-2.
MM101 detects IL-2 which has a predicted molecular weight of approximately 17 kDa.
The MM101 mouse IL2 antibody (clone 5H4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM102B (biotinylated conjugate of clone 1A12). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM101 (clone 5H4) and biotinylated antibody MM102B (clone 1A12) have successfully been used in combination with recombinant mouse IL2 protein SMIL2 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An atypical CD8 T-cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin-13.
MM101 was used in ELISA to study the mechanisms underlying CD8 T-cell-mediated pathology during genital tract Chlamydia infections and the significance of IL-13-producing cells
|Johnson RM,Kerr MS,Slaven JE||Immunology (142:248)||2014|
PmpG303-311, a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice.
MM101 was used in ELISA to study a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice
|Johnson RM,Yu H,Kerr MS,Slaven JE,Karunakaran KP,Brunham RC||Infection and immunity (80:2204)||2012|
Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract.
MM101 was used in ELISA to investigate Plac8-dependent and iNOS-dependent mechanisms of clearing Chlamydia muridarum genital tract infections
|Johnson RM,Kerr MS,Slaven JE||Journal of immunology (Baltimore, Md. : 1950) (188:1896)||2012|
Toxoplasma gondii glycosylphosphatidylinositols up-regulate major histocompatibility complex (MHC) molecule expression on primary murine macrophages.
MM101 was used in ELISA to study the effect of T. gondii glycosylphosphatidylinositols on MHC expression in macrophages
|Debierre-Grockiego F,Molitor N,Schwarz RT,Lüder CG||Innate immunity (15:25)||2009|
Up-regulation of IL-4 production by the activated cAMP/cAMP-dependent protein kinase (protein kinase A) pathway in CD3/CD28-stimulated naive T cells.
MM101 was used in ELISA to investigate the role of cAMP/PKA pathway for the modulation of interleukin 4 expression in T cells
|Tokoyoda K,Tsujikawa K,Matsushita H,Ono Y,Hayashi T,Harada Y,Abe R,Kubo M,Yamamoto H||International immunology (16:643)||2004|
Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
MM101 was used in ELISA to study the mechanism for T cell differentiation
|Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A||Journal of immunology (Baltimore, Md. : 1950) (161:2825)||1998|
Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.
MM101 was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression
|Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M||The Journal of pharmacology and experimental therapeutics (286:767)||1998|
Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.
MM101 was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system
|Ichinose T,Takano H,Miyabara Y,Sagai M||Toxicological sciences : an official journal of the Society of Toxicology (44:70)||1998|
Murine strain differences in airway inflammation caused by diesel exhaust particles.
MM101 was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants
|Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M||The European respiratory journal (11:291)||1998|
Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.
MM101 was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression
|Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M||American journal of respiratory and critical care medicine (156:36)||1997|
Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.
MM101 was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation
|Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E||Journal of immunology (Baltimore, Md. : 1950) (157:566)||1996|
Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.
MM101 was used in blocking/activating experiment to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells
|DeKruyff RH,Fang Y,Umetsu DT||Journal of immunology (Baltimore, Md. : 1950) (160:2231)||1998|
interleukin-2; T-cell growth factor; TCGF