Description: This monoclonal RN28 antibody reacts with IL-28 (IFN lambda), a member of the IFN-lambda family which mediates the induction of anti-viral protection in a wide variety of cells.
Applications Reported: The RN28 antibody has been reported for use in ELISA capture of mouse IL-28.
Applications Tested: This RN28 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-28 in combination with the biotin polyclonal (cat. 13-7282) antibody for detection. A suitable range of concentrations of this antibody for ELISA capture is 1.0-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
IL28 is a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IFN-lambda-2; IFN-lambda-3; IFN-lambda2; IL-28A; IL-28B; interferon alpha; Interferon lambda-2; Interferon lambda-3; interferon-lambda2; interleukin 28; interleukin 28A; interleukin 28B; Interleukin-28A; Interleukin-28B
Gene Aliases: EG330496; IFL-1; Ifnl2; Ifnl3; IL-28A; IL-28B; Il28; Il28a; Il28b; INF-alpha; INF-lambda