Description: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.
Applications Reported: MP5-32C11 has been reported for use in detection of mouse IL-6 by ELISA and ELISPOT.
Applications Tested: The MP5-32C11 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the purified MP5-20F3 (14-7061) antibody for capture and recombinant mouse IL-6 (14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; BSF2; CTL differentiation factor; HGF; HSF; Hybridoma growth factor; IFNB2; IL-6; Interferon beta-2; interleukin HP-1; Interleukin-6
Gene Aliases: Il-6
Entrez Gene ID: (Mouse) 16193