Invitrogen

IL-6 Monoclonal Antibody (MP5-32C11), Biotin, Functional Grade, eBioscience™

Advanced Verification

7 Published Figures

7 References

View all (218) IL-6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (7)
Advanced Verification (1)

FIGURE: 1 / 8

IL-6 Antibody (36-7062-85) in ELISA

Journal of biomedical science 2021 - Fig. 6 5-MTP suppresses HFD-induced IL-6 and HSP60 in the atherosclerotic lesions of ApoE -/- mice. Chow diet-fed or HFD-fed ApoE -/- mice were treated with saline or L-5-MTP twice weekly for 20 wk. ( A , B ) IL-6 and ( C , D ) OPG in paraffin-embedded aortic tissues were analyzed by immunohistochemistry. Negative control represents staining with a 2nd antibody. Representative microphotographs of the immuno... View More

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IL-6 Antibody — Figure 1 Acox1 Lampe1 mutation promotes proinflammatory immune responsiveness. (A and B) Twelve-week-old, chow diet-fed, Acox1 Lampe1 mice and WT littermate controls were challenged i.p. with biotinylated capture Ab (20 mug/mouse) 3 hours prior to i.v. saline (NS) or LPS challenge (25 mug/mouse; ultrapure E. coli 0111:B4). Twenty-four hours later, serum was collected, and IL-6, TNF-alpha, IFN-gamma, and IL-17A levels were quantified by IVCCA ELISA. (C) Supernatant IL-6 and IL-1beta levels from BM-derived DCs (BMDC), of chow diet-fed, Acox1 Lampe1 mice and WT littermate controls stimulated for 24 hours with saline (NS) or LPS (100 ng/mL). (D) Splenic naive CD4 + T cells from chow diet-fed WT mice, isolated by negative selection using magnetic beads, were cultured under Th17 and Th1 polarizing conditions with or without peroxisomal beta-oxidation inhibitor (thioridizine, 1 muM) and the frequency of IL-17A- and IFN-gammaexpressing cells were quantified by flow cytometry. (E and F) Splenic naive CD4 + T cells from 12-week-old, chow diet-fed, Acox1 Lampe1 mice and WT littermate controls, isolated by negative selection using magnetic beads, were cultured under Th17 and Th1 polarizing conditions, and the frequency of IL-17A- and IFN-gamma-expressing cells was quantified by flow cytometry. (G) Frequency of total splenic Th1 (CD3 + CD4 + IFN-gamma + IL-17A -), Th17 (CD3 + CD4 + IL-17A + IFN-gamma -), and Treg (CD3 + CD4 + FOXP3 +) in chow diet-fe {TM}

Product Details

36-7062-85

Applications

Tested Dilution

Publications

ELISA (ELISA)

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

MP5-32C11

Conjugate

Biotin , Functional Grade Biotin , Functional Grade Biotin , Functional Grade

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469761

Product Specific Information

Description: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: The Functional Grade Biotin anti-mouse IL-6 antibody (clone MP5-32C11) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New Yorkz.

Applications Tested: The Functional Grade Biotin MP5-32C11 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-6 in combination with the purified MP5-20F3 (14-7061) antibody for capture and recombinant mouse IL-6 (14-8061) as the standard.A suitable range of concentrations of the FG Biotin MP5-32C11 antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8.0 pg/mL should be included in each ELISA plate.

Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.