Invitrogen

IL-6 Monoclonal Antibody (MP5-32C11), Biotin, Functional Grade, eBioscience™

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

6 References

View all (145) IL-6 antibodies

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IL-6 Antibody in ELISA (ELISA) — Fig. 6 5-MTP suppresses HFD-induced IL-6 and HSP60 in the atherosclerotic lesions of ApoE -/- mice. Chow diet-fed or HFD-fed ApoE -/- mice were treated with saline or L-5-MTP twice weekly for 20 wk. ( A , B ) IL-6 and ( C , D ) OPG in paraffin-embedded aortic tissues were analyzed by immunohistochemistry. Negative control represents staining with a 2nd antibody. Representative microphotographs of the immunohistochemical staining for ( A ) IL-6 and ( C ) OPG are shown. Immunopositive areas of ( B ) IL-6 and ( D ) OPG in aorta tissues were quantified as a percentage of the total aortic area in each section. Data represent mean +- SEM (Chow + saline group: n = 6, HFD + saline group: n = 6 and HFD + 5-MTP group: n = 5). E Plasma IL-6 levels in HFD-fed ApoE -/- mice with injection of saline (n = 19) or L-5-MTP (n = 9) twice weekly for 20 wk for were measured by ELISA. Data represent mean +- SEM. After pretreating VSMCs with DMSO, DL-5-MTP, or L-5-MTP (100 muM) for 30 min, VSMCs were treated with vehicle or Pam3CSK4 (Pam3) in calcifying medium for 24 h. F IL6 and G OPG level in culture supernatants was measured by ELISA. Data represent mean +- SD of at least 3 independent experiments. HSP60 contents in aorta tissues from ApoE -/- mice fed chow diet with saline (n = 5) or HFD with saline (n = 5) or L-5-MTP (n = 5) for 20 wk were analyzed by immunohistochemistry. Representative microphotographs of the immunohistochemical staining ( H ) and quantification of immunopositive positive ar

IL-6 Antibody — Figure 1 Open in a separate window Acox1 Lampe1 mutation promotes proinflammatory immune responsiveness. ( A and B ) Twelve-week-old, chow diet-fed, Acox1 Lampe1 mice and WT littermate controls were challenged i.p. with biotinylated capture Ab (20 mug/mouse) 3 hours prior to i.v. saline (NS) or LPS challenge (25 mug/mouse; ultrapure E. coli 0111:B4). Twenty-four hours later, serum was collected, and IL-6, TNF-alpha, IFN-gamma, and IL-17A levels were quantified by IVCCA ELISA. ( C ) Supernatant IL-6 and IL-1beta levels from BM-derived DCs (BMDC), of chow diet-fed, Acox1 Lampe1 mice and WT littermate controls stimulated for 24 hours with saline (NS) or LPS (100 ng/ml). ( D ) Splenic naive CD4 + T cells from chow diet-fed WT mice, isolated by negative selection using magnetic beads, were cultured under Th17 and Th1 polarizing conditions with or without peroxisomal beta-oxidation inhibitor (thioridizine, 1 muM) and the frequency of IL-17A- and IFN-gammaexpressing cells were quantified by flow cytometry. ( E and F ) Splenic naive CD4 + T cells from 12-week-old, chow diet-fed, Acox1 Lampe1 mice and WT littermate controls, isolated by negative selection using magnetic beads, were cultured under Th17 and Th1 polarizing conditions, and the frequency of IL-17A- and IFN-gamma-expressing cells was quantified by flow cytometry. ( G ) Frequency of total splenic Th1 (CD3 + CD4 + IFN-gamma + IL-17A - ), Th17 (CD3 + CD4 + IL-17A + IFN-gamma - ), and Treg (CD3 + CD4 + FOXP3 + ) in chow diet-fe {TM}

Product Details

36-7062-85

Applications	Tested Dilution	Publications
ELISA (ELISA)	-	View 5 publications 5 publications
Functional Assay (FN)	Assay-Dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	MP5-32C11
Conjugate	Biotin , Functional Grade
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_469761

Product Specific Information

Description: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: The Functional Grade Biotin anti-mouse IL-6 antibody (clone MP5-32C11) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New Yorkz.

Applications Tested: The Functional Grade Biotin MP5-32C11 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-6 in combination with the purified MP5-20F3 (14-7061) antibody for capture and recombinant mouse IL-6 (14-8061) as the standard.A suitable range of concentrations of the FG Biotin MP5-32C11 antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8.0 pg/mL should be included in each ELISA plate.

Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Target Information

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE^-/- mice fed high fat diet.

Journal of biomedical science

Lee GL,Liao TL,Wu JY,Wu KK,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 36-7062-85) in ELISA

Mon Nov 08 00:00:00 EST 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Published figure using IL-6 monoclonal antibody (Product # 36-7062-85) in ELISA

Fri Jul 02 00:00:00 EDT 2021

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 36-7062-85) in ELISA

Wed Dec 04 00:00:00 EST 2019

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-6 monoclonal antibody (Product # 36-7062-85) in ELISA

Wed May 29 00:00:00 EDT 2019

Peroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease.

JCI insight

Moreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S

36-7062 was used in Functional assays to demonstrate that β-oxidation links metabolism and immune responsiveness.

Thu Mar 22 00:00:00 EDT 2018

Peroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease.

JCI insight

Moreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S

36-7062 was used in Functional assays to demonstrate that β-oxidation links metabolism and immune responsiveness.

Thu Mar 22 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

Gene Aliases: Il-6; Il6

UniProt ID: (Mouse) P08505

Entrez Gene ID: (Mouse) 16193

Function(s)

Process(es)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

IL-6 Monoclonal Antibody (MP5-32C11), Biotin, Functional Grade, eBioscience™

Product Details

36-7062-85

ELISA (ELISA)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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