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Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
ELISA (ELISA) |
- | View 5 publications 5 publications |
Functional Assay (FN) |
Assay-Dependent | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Rat / IgG2a, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
MP5-32C11 |
Conjugate |
Biotin , Functional Grade |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
no preservative |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_469761 |
Description: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.
Applications Reported: The Functional Grade Biotin anti-mouse IL-6 antibody (clone MP5-32C11) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.
For detailed methodology in this application, please refer to Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New Yorkz.
Applications Tested: The Functional Grade Biotin MP5-32C11 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-6 in combination with the purified MP5-20F3 (14-7061) antibody for capture and recombinant mouse IL-6 (14-8061) as the standard.A suitable range of concentrations of the FG Biotin MP5-32C11 antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8.0 pg/mL should be included in each ELISA plate.
Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.
Storage and handling: Use in a sterile environment.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: Il-6; Il6
UniProt ID: (Mouse) P08505
Entrez Gene ID: (Mouse) 16193
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