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Invitrogen
IL-9 Monoclonal Antibody (D9302C12), Functional Grade, eBioscience™
Product Details
16-7093-85
| Applications | Tested Dilution | Publications |
|---|---|---|
Neutralization (Neu) |
Assay-Dependent | View 1 publication 1 publication |
Functional Assay (FN) |
Assay-Dependent | - |
Inhibition Assays (IA) |
- | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Armenian hamster / IgG |
Recommended Isotype Control |
Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
D9302C12 |
Conjugate |
Functional Grade |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
no preservative |
Storage conditions |
4° C |
RRID |
AB_11043136 |
Product Specific Information
Description: This monoclonal D9302C12 antibody reacts with and inhibits the bioactivity of mouse IL-9. IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.
Applications Reported: The D9302C12 antibody inhibits the bioactivity of mouse IL-9.
Applications Tested: The ND50 of D9302C12, as measured by the inhibition TS-1 cell proliferation, is less than or equal to 500 ng/mL in the presence of 0.1 ng/mL of Mouse IL-9 Recombinant Protein. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. This antibody should be carefully titrated for optimal performance in the assay of interest.
Bioactivity: The ND50 of D9302C12, as measured by the inhibition TS-1 cell proliferation, is less than or equal to 500 ng/mL in the presence of 0.1 ng/mL of Mouse IL-9 Recombinant Protein.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytokine P40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; T-cell growth factor P40
Gene Aliases: Il-9; Il9; P40
UniProt ID: (Mouse) P15247
Entrez Gene ID: (Mouse) 16198
Molecular Function:
interleukin superfamily
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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