Spectral confocal microscopy of CHO cells using MA1-17751. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of MA1-17751 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||cDNA encoding human IL13-R alpha 1 (extracellular domain)|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:200 - 1:400|
|Flow Cytometry (Flow)||1.2 ug/10^6 cells|
|Immunocytochemistry (ICC)||1ug/10^6 cells|
|Immunofluorescence (IF)||1 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes native IL13R protein expressed in transfected mammalian cells.
Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and im-mune responses. IL-13Ra1 together with IL-4Ra forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.