Invitrogen

IRF4 Monoclonal Antibody (3E4), eBioscience™

Product Image Gallery

IRF4 Antibody — Figure 3 Targeted ROCK2 inhibition decreased the binding of ROCK2 to the Irf4 and Bcl6 promoters at Th17-skewing conditions. Human peripheral blood CD4 + T cells were stimulated under Th17-skewing conditions for 48 hours in the absence or presence of KD025, chromatin was purified and proceeded to ChIP-qPCR analyses with anti-ROCK2 or anti-STAT3 antibodies ( a , b ), or Cells were stained with antibodies to IRF4 and analyzed by Flow Cytometry ( c , d ). ( c ) IRF4 expression measured by flow cytometry. Left, Mean Fluorescence Index (MFI) for IRF4 is plotted as percentage of MFI for skewed cells (as 100%). Right, Histogram of the flow cytometry analysis. The average of four different experiments is shown. *p < 0.05; **p < 0.01.

IRF4 Antibody in Immunoblot (IB) — Immunoblotting of reduced cell lysate prepared from mouse splenocytes with 5 µg/mL of Anti-Human/Mouse IRF4 antibody.Splenocytes were cultured for 3 days in either complete media alone (Lane 1) or polarized under Th2 conditions (Lane 2).Bands were visualized using Anti-Mouse IgG HRP.

Product Details

Tested Applications

Dilution

Western Blot (WB)

5 µg/mL

Published Applications

Flow Cytometry (Flow)

See 10 publications below

ChIP assay (ChIP)

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3E4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10804654

Product Specific Information

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), T helper (Th)2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Applications Reported: This 3E4 antibody has been reported for use in western blotting.

Applications Tested: This 3E4 antibody has been tested by western blot of reduced cell lysate prepared from splenocytes cultured for 3 days in the presence of plate-bound Anti-Mouse CD3 and soluble Anti-Mouse CD28. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

Gene Aliases: AI385587; IRF-4; IRF4; LSIRF; MUM1; NF-EM5; SHEP8; Spip

UniProt ID: (Human) Q15306, (Mouse) Q64287

Entrez Gene ID: (Human) 3662, (Mouse) 16364

Molecular Function: nucleic acid binding transcription factor