Invitrogen

IRF4 Monoclonal Antibody (3E4), eBioscience™

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IRF4 Antibody (14-9858-82) in WB

Western blot was performed using Anti-IRF4 Monoclonal Antibody (3E4) (Product # 14-9858-82) and a 51kDa band corresponding to IRF4 was observed in IM9 and was absent in MOLT-4, Jurkat and U-937 as reported. Whole cell extracts (30 µg lysate) of IM9 (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3) and U-937 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The b... View More

IRF4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IRF4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of IRF4 was observed in IM9 in comparison to MOLT-4, Jurkat and U-937 using Anti-IRF4 Monoclonal antibody (3E4) (Product # 14-9858-82) in Western Blot. {RE}

Product Details

14-9858-82

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 19 publications 19 publications

ELISA (ELISA)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3E4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10804654

Product Specific Information

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), T helper (Th)2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Applications Reported: This 3E4 antibody has been reported for use in western blotting.

Applications Tested: This 3E4 antibody has been tested by western blot of reduced cell lysate prepared from splenocytes cultured for 3 days in the presence of plate-bound Anti-Mouse CD3 and soluble Anti-Mouse CD28. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation.

The Journal of experimental medicine

Schmitt P,Duval A,Camus M,Lefrançais E,Roga S,Dedieu C,Ortega N,Bellard E,Mirey E,Mouton-Barbosa E,Burlet-Schiltz O,Gonzalez-de-Peredo A,Cayrol C,Girard JP

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Mon Jun 03 00:00:00 UTC 2024

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Wed Jul 05 00:00:00 UTC 2023

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Tue Aug 24 00:00:00 UTC 2021

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Tue Feb 11 00:00:00 UTC 2020

Pro-Survival Lipid Sphingosine-1-Phosphate Metabolically Programs T Cells to Limit Anti-tumor Activity.

Cell reports

Chakraborty P,Vaena SG,Thyagarajan K,Chatterjee S,Al-Khami A,Selvam SP,Nguyen H,Kang I,Wyatt MW,Baliga U,Hedley Z,Ngang RN,Guo B,Beeson GC,Husain S,Paulos CM,Beeson CC,Zilliox MJ,Hill EG,Mehrotra M,Yu XZ,Ogretmen B,Mehrotra S

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in ELISA

Tue Aug 13 00:00:00 UTC 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Wed Mar 06 00:00:00 UTC 2019

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Mon Jan 31 00:00:00 UTC 2022

Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3⁺RORγt⁺ Regulatory T Cells.

Cell reports

Ogawa C,Bankoti R,Nguyen T,Hassanzadeh-Kiabi N,Nadeau S,Porritt RA,Couse M,Fan X,Dhall D,Eberl G,Ohnmacht C,Martins GA

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in ChIP assay

Tue Oct 02 00:00:00 UTC 2018

ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells.

Scientific reports

Chen W,Nyuydzefe MS,Weiss JM,Zhang J,Waksal SD,Zanin-Zhorov A

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Fri Nov 09 00:00:00 UTC 2018

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Ubiquitin specific peptidase 4 stabilizes interferon regulatory factor protein and promotes its function to facilitate interleukin-4 expression in T helper type 2 cells.

International journal of molecular medicine

Guo Z,Xu P,Ge S,Zhang C,Zheng X,Xu J,Liu Z,Li B,Ge S

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Western Blot

Sun Oct 01 00:00:00 UTC 2017

Ubiquitin specific peptidase 4 stabilizes interferon regulatory factor protein and promotes its function to facilitate interleukin-4 expression in T helper type 2 cells.

International journal of molecular medicine

Guo Z,Xu P,Ge S,Zhang C,Zheng X,Xu J,Liu Z,Li B,Ge S

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Western Blot

Sun Oct 01 00:00:00 UTC 2017

RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization.

Cell death & disease

Meng YH,Zhou WJ,Jin LP,Liu LB,Chang KK,Mei J,Li H,Wang J,Li DJ,Li MQ

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Thu Oct 12 00:00:00 UTC 2017

STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides.

Clinical cancer research : an official journal of the American Association for Cancer Research

Vieyra-Garcia PA,Wei T,Naym DG,Fredholm S,Fink-Puches R,Cerroni L,Odum N,O'Malley JT,Gniadecki R,Wolf P

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Immunohistochemistry

Fri Jul 01 00:00:00 UTC 2016

Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis.

Mucosal immunology

Mohapatra A,Van Dyken SJ,Schneider C,Nussbaum JC,Liang HE,Locksley RM

14-9858 was used in Flow cytometry/Cell sorting to study how ILC2s link alveolar function to regulation of airway flow, revealing a key interaction between resident lymphoid and structural cells that might underlie similar organisational hierarchies in other organs.

Fri Jan 01 00:00:00 UTC 2016

γ-Tocopherol supplementation of allergic female mice augments development of CD11c+CD11b+ dendritic cells in utero and allergic inflammation in neonates.

American journal of physiology. Lung cellular and molecular physiology

Abdala-Valencia H,Soveg F,Cook-Mills JM

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Mon Jul 04 00:00:00 UTC 2016

Co-potentiation of antigen recognition: A mechanism to boost weak T cell responses and provide immunotherapy in vivo.

Science advances

Hoffmann MM,Molina-Mendiola C,Nelson AD,Parks CA,Reyes EE,Hansen MJ,Rajagopalan G,Pease LR,Schrum AG,Gil D

14-9858 was used in Flow cytometry/Cell sorting to investigate co-potentiation, showing that weak T-cell responses can be boosted to provide immunotherapy in vivo.

Thu Oct 01 00:00:00 UTC 2015

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Sun Mar 15 00:00:00 UTC 2015

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Thu Jan 01 00:00:00 UTC 2015

Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice.

Nature immunology

Watchmaker PB,Lahl K,Lee M,Baumjohann D,Morton J,Kim SJ,Zeng R,Dent A,Ansel KM,Diamond B,Hadeiba H,Butcher EC

14-9858 was used in Flow cytometry/Cell sorting to characterise human gut dendritic cell populations, and define their relationship to previously studied human and mouse dendritic cells.

Wed Jan 01 00:00:00 UTC 2014

OX40 signaling favors the induction of T(H)9 cells and airway inflammation.

Nature immunology

Xiao X,Balasubramanian S,Liu W,Chu X,Wang H,Taparowsky EJ,Fu YX,Choi Y,Walsh MC,Li XC

14-9858 was used in Flow cytometry/Cell sorting to identify a previously unknown mechanism for the induction of Th9 cells, with potential clinical implications in allergic inflammation.

Mon Oct 01 00:00:00 UTC 2012

Regulatory T-cell suppressor program co-opts transcription factor IRF4 to control T(H)2 responses.

Nature

Zheng Y,Chaudhry A,Kas A,deRoos P,Kim JM,Chu TT,Corcoran L,Treuting P,Klein U,Rudensky AY

Published figure using IRF4 monoclonal antibody (Product # 14-9858-82) in Flow Cytometry

Thu Mar 19 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

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Gene Aliases: AI385587; IRF-4; IRF4; LSIRF; MUM1; NF-EM5; SHEP8; Spip

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UniProt ID: (Human) Q15306, (Mouse) Q64287

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Entrez Gene ID: (Human) 3662, (Mouse) 16364

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein-lysine N-methyltransferase activity sequence-specific DNA binding regulatory region DNA binding DNA binding winged helix/forkhead transcription factor

Process(es)

transcription from RNA polymerase II promoter peptidyl-lysine methylation negative regulation of toll-like receptor signaling pathway T cell activation defense response to protozoan myeloid dendritic cell differentiation positive regulation of DNA binding histone H3 acetylation histone H4 acetylation positive regulation of interleukin-10 biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-4 biosynthetic process regulation of T-helper cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway T-helper 17 cell lineage commitment transcription, DNA-templated regulation of transcription, DNA-templated protein methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRF4 Monoclonal Antibody (3E4), eBioscience™

IRF4 Antibody (14-9858-82) in WB

Product Details

14-9858-82

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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