|Tested species reactivity||Bovine, Dog, Human, Mouse, Pig, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified NTF97 from bovine erythrocytes.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/test|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 5 publications below|
|Western Blot (WB)||See 4 publications below|
|Blocking Assay (BLOCK)||See 3 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
MA3-070 detects Importin beta 1 from human, canine, bovine, rat and mouse tissues as well as porcine cell lysates. Importin beta 1 is also known as NTF97, Nuclear Transport Factor p97, and karyopherin beta 1. This antibody does not detect bacterially expressed recombinant NTF97.
MA3-070 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~97 kDa protein representing NTF97 in MDBK cell homogenate. Immunofluorescence staining of NTF97 in MDBK cells with MA3-070 results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE. This antibody can inhibit import of nuclear localization sequence-containing proteins in permeabilized cells.
The MA3-070 antigen is purified NTF97 from bovine erythrocytes.
The accumulation of proteins in the nucleus is mediated by short sequences of basic amino acids called nuclear localization sequences (NLSs). These sequences are necessary and sufficient to direct a protein or inert carrier to the nuclear interior. Nuclear protein import is accomplished by two sequential, energy dependent events. The first step, docking at the nuclear pore complex, requires a 54/56 kDa nuclear localization signal receptor (a-karyopherin, importin-a, SRP-a) and the nuclear transport factor p97 (NTF97, b-karyopherin, importin-b). The second step, translocation across the nuclear envelope (NE), requires the GTP-binding protein, Ran/TC4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
MA3-070 was used in immunocytochemistry to study the role of 4EBP1 in the mechanism by which sodium selenite induces non-canonical stress granules and the implications for the chemotherapeutic use of selenite
|Fujimura K,Sasaki AT,Anderson P||Nucleic acids research (40:8099)||2012|
Modulation of integrin-linked kinase nucleo-cytoplasmic shuttling by ILKAP and CRM1.
MA3-070 was used in immunocytochemistry to study the role of ILKAP and CRM-1 in modulating the movement of integrin-linked kinase between the nucleus and the cytoplasm
|Nakrieko KA,Vespa A,Mason D,Irvine TS,D'Souza SJ,Dagnino L||Cell cycle (Georgetown, Tex.) (7:2157)||2008|
HuD distribution changes in response to heat shock but not neurotrophic stimulation.
MA3-070 was used in immunocytochemistry to investigate the changes of HuD distribution during heat shock and neurotrophic stimulation
|Burry RW,Smith CL||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (54:1129)||2006|
Sugar-dependent nuclear import of glycosylated proteins in living cells.
MA3-070 was used in immunocytochemistry to compare the nuclear localization signal-mediated nuclear import pathway with the sugar-dependent nuclear import pathway
|Rondanino C,Bousser MT,Monsigny M,Roche AC||Glycobiology (13:509)||2003|
Herpes simplex virus type 1 entry into host cells: reconstitution of capsid binding and uncoating at the nuclear pore complex in vitro.
MA3-070 was used in immunocytochemistry to study the entry of herpes simplex type 1 virus into host cells.
|Ojala PM,Sodeik B,Ebersold MW,Kutay U,Helenius A||Molecular and cellular biology (20:4922)||2000|
Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin.
MA3-070 was used in western blot to investigate the role of importin beta in intracellular protein degradation process
|Zhong Y,Wang Y,Yang H,Ballar P,Lee JG,Ye Y,Monteiro MJ,Fang S||The Journal of biological chemistry (286:33921)||2011|
Localization of retinitis pigmentosa 2 to cilia is regulated by Importin beta2.
MA3-070 was used in western blot to investigate the regulatory role of importin beta2 in the cilial localization of retinitis pigmentosa 2
|Hurd TW,Fan S,Margolis BL||Journal of cell science (124:718)||2011|
Nuclear entry of activated MAPK is restricted in primary ovarian and mammary epithelial cells.
MA3-070 was used in western blot to study the alterations of MAPK activation and nuclear transport in primary ovarian and mammary epithelial cells
|Smith ER,Cai KQ,Smedberg JL,Ribeiro MM,Rula ME,Slater C,Godwin AK,Xu XX||PloS one (5:null)||2010|
The N-terminal domain of the mammalian nucleoporin p62 interacts with other nucleoporins of the FXFG family during interphase.
MA3-070 was used in western blot to study the interaction of mammalian nucleoporin p62 interacts with the FXFG family protein during interphase
|Stochaj U,Ba¿ski P,Kodiha M,Matusiewicz N||Experimental cell research (312:2490)||2006|
A novel mechanism of E2F1 regulation via nucleocytoplasmic shuttling: determinants of nuclear import and export.
MA3-070 was used in blocking or activating experiment to investigate the nucleocytoplasmic shuttling of E2F1
|Ivanova IA,Vespa A,Dagnino L||Cell cycle (Georgetown, Tex.) (6:2186)||2007|
Enhanced intracellular mobility and nuclear accumulation of DNA plasmids associated with a karyophilic protein.
MA3-070 was used in blocking or activating experiment to study the effect of nuclear localization signals on the intracellular transport of injected plasmid DNA
|Mesika A,Kiss V,Brumfeld V,Ghosh G,Reich Z||Human gene therapy (16:200)||2005|
Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro.
MA3-070 was used in blocking/activating experiment to study the modulatory mechanism of the nuclear export of NFAT
|Kehlenbach RH,Dickmanns A,Gerace L||The Journal of cell biology (141:863)||1998|
Multiple importins function as nuclear transport receptors for the Rev protein of human immunodeficiency virus type 1.
MA3-070 was used in chromatin immunoprecipitation to study the binding of Rev to the nuclear transport receptors importins and its nuclear import through several transport pathways.
|Arnold M,Nath A,Hauber J,Kehlenbach RH||The Journal of biological chemistry (281:20883)||2006|
An immunohistochemical approach to monitor the prolactin-induced activation of the JAK2/STAT5 pathway in pancreatic islets of Langerhans.
MA3-070 was used in immunohistochemistry to evaluate an immunohistochemical methodology for studying JAK2/STAT5 pathway in pancreatic islets of Langerhans
|Brelje TC,Svensson AM,Stout LE,Bhagroo NV,Sorenson RL||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (50:365)||2002|
Importin beta-mediated nuclear import of fibroblast growth factor receptor: role in cell proliferation.
MA3-070 was used in immunoprecipitation to study the role of importin beta in the nuclear import of fibroblast growth factor receptor.
|Reilly JF,Maher PA||The Journal of cell biology (152:1307)||2001|
Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta.
MA3-070 was used in immunoprecipitation to study the mechanism for the cyclin B1-Cdc2 import into the nucleus
|Takizawa CG,Weis K,Morgan DO||Proceedings of the National Academy of Sciences of the United States of America (96:7938)||1999|