Western blot analysis of Influenza A H1N1 NA (swine flu) immunizing peptide conjugated to a carrier protein using a Influenza A H1N1 NA polyclonal antibody (Product # PA5-23413) at 2 ug/ml. A band at ~29 kDa was detected, corresponding to the MW of the immunogen/fusion protein. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (238 CFTIMTDGPSNGQASY 253) of Influenza A H1N1 NA protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Influenza A H1N1 NA, also known as Neuraminidase, is a protein belonging to the Influenza A virus strain (strain A/Swine/New Jersey/11/1976 H1N1) with 469 amino acids. It belongs to the glycosyl hydrolase 34 family. This Influenza A virus has aves, human and pig as its host. Neuraminidase is a homotetramer located in the virion membrane and host apical cell membrane and preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. This protein is a receptor-destroying enzyme that catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates (cellular receptors) during virus budding to facilitate virus release, spreading, prevents self-aggregation of virus and ensures the efficient spread of the progeny virus from cell to cell. It may facilitate viral invasion of the upper airways by cleaving the sialic acid moieties on the mucin of the airway epithelial cells. Neuraminidase plays a role in the determination of host range restriction on replication and virulence.
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