Invitrogen

Insulin Monoclonal Antibody (7F8)

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Product Details

MA1-10517

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

ELISA (ELISA)

1-10 µg/mL
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Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7F8

Immunogen

Purified human Insulin.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076182

Product Specific Information

MA1-10517 detects Insulin from human, bovine, and porcine samples.

MA1-10517 has been successfully used in ELISA applications.

The MA1-10517 immunogen is purified human Insulin.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Ins; Insulin; preproinsulin; proinsulin

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Gene Aliases: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10

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UniProt ID: (Pig) P01315, (Human) Q5EEX2

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Entrez Gene ID: (Pig) 397415, (Bovine) 280829, (Human) 3630

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Function(s)
protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity identical protein binding protein binding growth factor
Process(es)
negative regulation of acute inflammatory response glucose metabolic process regulation of cellular amino acid metabolic process acute-phase response G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway lipid biosynthetic process glycoprotein biosynthetic process positive regulation of nitric oxide mediated signal transduction positive regulation of glucose metabolic process positive regulation of phosphatidylinositol 3-kinase signaling lactate biosynthetic process regulation of transmembrane transporter activity positive regulation of cell migration positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of NAD(P)H oxidase activity wound healing lipoprotein biosynthetic process negative regulation of protein catabolic process glucose homeostasis positive regulation of MAPK cascade negative regulation of gluconeogenesis positive regulation of fatty acid biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation negative regulation of protein secretion positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipid catabolic process positive regulation of lipoprotein lipase activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of protein localization to nucleus positive regulation of dendritic spine maintenance regulation of protein localization to plasma membrane negative regulation of reactive oxygen species biosynthetic process response to L-arginine neuron projection maintenance negative regulation of feeding behavior signal transduction feeding behavior protein secretion response to heat response to glucose positive regulation of gene expression response to nutrient levels positive regulation of insulin secretion response to food negative regulation of appetite positive regulation of mammary gland epithelial cell proliferation positive regulation of Rho protein signal transduction estradiol secretion negative regulation of apoptotic process glucose import in response to insulin stimulus positive regulation of protein secretion response to growth hormone positive regulation of peptide hormone secretion positive regulation of cell maturation negative regulation of lactation positive regulation of lactation positive regulation of blood circulation response to butyrate MAPK cascade regulation of transcription, DNA-templated ER to Golgi vesicle-mediated transport cell-cell signaling glucose transport positive regulation of cell growth positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization cellular protein metabolic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of vasodilation positive regulation of vasodilation positive regulation of lipid biosynthetic process regulation of protein secretion positive regulation of nitric-oxide synthase activity positive regulation of brown fat cell differentiation negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
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