|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant fragment of human ITGA5 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-15568 targets ITGA5 in FACS, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15568 immunogen is purified recombinant fragment of human ITGA5 expressed in E. Coli.
MA5-15568 detects ITGA5 which has a predicted molecular weight of approximately 114kDa.
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells.
MA5-15568 was used in immunohistochemistry and western blot investigate how integrins regulate adipose tissue stem cell proliferation and differentiation
|Morandi EM,Verstappen R,Zwierzina ME,Geley S,Pierer G,Ploner C||Scientific reports (6:null)||2016|