|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, Kappa|
|Immunogen||Full length recombinant IL-25|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
As a member of the IL-17 family, IL-25 has been identified as a pro-inflammatory cytokine which is produced by Th17 effector cells thus enabling the TH17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis (1-3). IL-25 appears to stimulate IL-8 production and itself appears to be activated through IL-23, IL-6, and TGF-ß (1). Studies of IL-25 expression and activation through NF-kB taken together with IL-17 production and Th17 polarization through TLR ligand activation further closely tie the IL-17 and Th17 family together with the TLR signaling network initiating and controlling immune responses (4-7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.