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|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rabbit, Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the mouse junctophilin-1 (JP-1, junctophilin type 1, JPH1) protein, which differs from rat by one non-conservative amino acid replacement|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||2-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.
40-5100 was used in western blot to test if sAnk and SERCA interact.
|Desmond PF,Muriel J,Markwardt ML,Rizzo MA,Bloch RJ||The Journal of biological chemistry (290:27854)||2015|
Ca2+-dependent proteolysis of junctophilin-1 and junctophilin-2 in skeletal and cardiac muscle.
40-5100 was used in western blot to study junctophilin proteolysis in skeletal muscle.
|Murphy RM,Dutka TL,Horvath D,Bell JR,Delbridge LM,Lamb GD||The Journal of physiology (591:719)||2013|
Oxysterols modulate calcium signalling in the A7r5 aortic smooth muscle cell-line.
40-5100 was used in immunocytochemistry to assess the effects of oxysterols on calcium signaling mechanisms in A7r5 cells.
|Hammoud Y,Rice T,Mackrill JJ||Biochimie (95:568)||2013|
The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo.
40-5100 was used in immunohistochemistry to study the role of endothelial cells in myofiber differentiation and the development of bioengineered muscle tissue in vivo.
|Criswell TL,Corona BT,Wang Z,Zhou Y,Niu G,Xu Y,Christ GJ,Soker S||Biomaterials (34:140)||2013|
Further development of a tissue engineered muscle repair construct in vitro for enhanced functional recovery following implantation in vivo in a murine model of volumetric muscle loss injury.
40-5100 was used in immunohistochemistry - paraffin section and western blot to optimize tissue engineered muscle repair constructs.
|Corona BT,Machingal MA,Criswell T,Vadhavkar M,Dannahower AC,Bergman C,Zhao W,Christ GJ||Tissue engineering. Part A (18:1213)||2012|
|Human||Not Cited||Junctophilin 1 and 2 proteins interact with the L-type Ca2+ channel dihydropyridine receptors (DHPRs) in skeletal muscle.||Golini L,Chouabe C,Berthier C,Cusimano V,Fornaro M,Bonvallet R,Formoso L,Giacomello E,Jacquemond V,Sorrentino V||The Journal of biological chemistry (286:43717)||2011|
JP1; junctophilin type 1; junctophilin type1; junctophilin-1; mitsugumin72
ENSMUSG00000054314; JP-1; Jp1; Jph1