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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Guinea pig / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
GST fusion protein corresponding to residues 1097-1196 of mouse KCNMA1variant 2 (Intracellular, C-terminus) |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
0.8 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 1% BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2857209 |
Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
GST fusion protein sequence is: SHSSHSSQS SSKKSSSVHS IPSTANRPNR PKSRESRDKQ NATRMTRMGQ AEKKWFTDEP DNAYPRNIQI KPMSTHMANQ INQYKSTSSL IPPIREVEDE C
Homology: Mouse - 21/21 amino acid residues identical to the canonical sequence (AccessionQ08460); rat - 20/21 amino acid residues identical to the canonical sequence (AccessionQ62976); human - 19/21 amino acid residues identical to the canonical sequence (AccessionQ12791).
Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: big potassium channel alpha subunit; BK channel; BK channel alpha subunit; BKCA alpha; BKCA alpha subunit; calcium-activated potassium channel alpha subunit; Calcium-activated potassium channel subunit alpha-1; Calcium-activated potassium channel, subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; Maxi K channel; maxi-K channel HSLO; MaxiK; mSlo1; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; Slo homolog; Slo-alpha; Slo1; Slowpoke homolog; stretch-activated Kca channel
Gene Aliases: 5730414M22Rik; bA205K10.1; BKCa; BKTM; hSlo; KCa1.1; KCNMA; KCNMA1; KCNMA1b; KCNMA1c; MaxiK; mSlo; mSLO1; SAKCA; SLO; SLO-ALPHA; SLO1
UniProt ID: (Human) Q12791, (Mouse) Q08460, (Rat) Q7TQ56
Entrez Gene ID: (Human) 3778, (Mouse) 16531, (Rat) 83731
Molecular Function:
ion channel
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