|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant fragment of human KLHL25 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15656 targets KLHL25 in WB applications and shows reactivity with Human samples.
The MA5-15656 immunogen is purified recombinant fragment of human KLHL25 expressed in E. Coli.
MA5-15656 detects KLHL25 which has a predicted molecular weight of approximately 65kDa.
KLHL25 (ectoderm-neural cortex protein 2, ENC2) is a cytoplasmic protein that contains six Kelch regions and a single BTB (POZ) domain. KLHL25 is highly homologus to another Kelch-like protein, ENC1, and it is believed to operate in a manner similar to other Kelch-domain containing proteins. Kelch-domain repeat containing proteins often act as modifiers of Actin fibers. Expressed early in embryogenesis, ENC1 helps to mediate neuronal process formation. It also appears to have a role in neural crest cell differentiation. KLHL25 likely functions as a substrate specific adapter for protein ubiquitinating complexes. KLHL25 is expressed in most tissues with highest expression in brain and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.