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| Applications | Tested Dilution | Publications |
|---|---|---|
Immunohistochemistry (Frozen) (IHC (F)) |
Assay-dependent | - |
Flow Cytometry (Flow) |
1/50 - 1/100 | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
B199.2 |
Immunogen |
Proprietary Immunogen. |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS |
Contains |
0.09% sodium azide |
Storage conditions |
4°C or -20°C if preferred |
RRID |
AB_927716 |
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A.
KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-type lectin domain family 5 member B; CD161; HNKR-P1a; Killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; MGC138614; natural killer cell receptor; Natural killer cell surface protein P1A; NKR-P1A
Gene Aliases: CD161; CLEC5B; hNKR-P1A; KLRB1; NKR; NKR-P1; NKR-P1A; NKRP1A
UniProt ID: (Human) Q12918
Entrez Gene ID: (Human) 3820
Molecular Function:
transmembrane signal receptor
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