Rockland

Kinesin-1 Polyclonal Antibody

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Kinesin-1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of Rabbit anti-Kinesin-1. Tissue: Human Brain at 40X at pH 6.

Kinesin-1 Antibody in Western Blot (WB) — Western Blot of Rabbit anti-Kinesin 1 Antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 µg of Mouse Brain lysate. Lane 3: 10 µg of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:40,000 for 1hr at RT. Block: 5% BLOTTO 1 hr at RT. Predicted/Observed size: 72 kDa, ~70 kDa for kinesin-1. Other band(s): degradation.

Kinesin-1 Antibody — SDS-PAGE of Truncated Kinesin 1 Protein. Lane 1: Truncated Kinesin-1 Protein. Lane 2: none. Load: 5 µg per lane. Primary antibody: none. Secondary antibody: none. Block: Coomassie blue for 20 min, destain overnight at RT. Predicted/Observed size: 72 kDa, 72kDa for kinesin-1. Other band(s): degradation.

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:10,000

Immunohistochemistry (IHC)

Assay-dependent

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:100-1:500

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

77 mg/mL

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Kinesin-1 is directed against the human kinesin-1 protein. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.

Target Information

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Conventional kinesin heavy chain; epididymis secretory protein Li 61; kinesin 1 (110-120kD); kinesin heavy chain; Kinesin-1 heavy chain; U-KHC; Ubiquitous kinesin heavy chain; UKHC

Gene Aliases: HEL-S-61; KIF5B; KINH; KNS; KNS1; UKHC

UniProt ID: (Human) P33176

Entrez Gene ID: (Human) 3799

Molecular Function: cytoskeletal protein microtubule binding motor protein microtubule family cytoskeletal protein