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|Tested species reactivity||Mouse|
|Published species reactivity||Rabbit, Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C S(780) G L T L E P P G D P P P(792)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-641 detects kinesin C2 (KIFC2) from mouse tissues.
PA1-641 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing KIFC2 from mouse brain homogenate.
PA1-641 immunizing peptide corresponds to amino acid residues 780-792 from mouse KIFC2. PA1-641 immunizing peptide (Cat. # PEP-041) is available for use in neutralization and control experiments.
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure which includes a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. An expansive phylogenetic tree of kinesins and kinesin-related proteins (KRPs) has been assembled based on this information.
Kinesin C2 (KIFC2) is a C-type kinesin based on the position of the motor domain towards the carboxyl-terminus of the protein and is similar to members of the karyogamy protein 3 (KAR3) family involved in cell division. KIFC2 protein expression has been demonstrated in axons and dendrites of the central and peripheral nervous system. Unlike other C-type motor proteins, KIFC2 appears to play a role in retrograde axonal transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutant SOD1 impairs axonal transport of choline acetyltransferase and acetylcholine release by sequestering KAP3.
PA1-641 was used in western blot to study the role of a mutant SOD1 in impaired axonal transport of choline acetyltransferase and acetylcholine
|Tateno M,Kato S,Sakurai T,Nukina N,Takahashi R,Araki T||Human molecular genetics (18:942)||2009|
Microtubule-dependent movement of late endocytic vesicles in vitro: requirements for Dynein and Kinesin.
PA1-641 was used in immunohistochemistry to investigate the mechanism of the movement of late endocytic vesicles.
|Bananis E,Nath S,Gordon K,Satir P,Stockert RJ,Murray JW,Wolkoff AW||Molecular biology of the cell (15:3688)||2004|
KIFC2 is a novel neuron-specific C-terminal type kinesin superfamily motor for dendritic transport of multivesicular body-like organelles.
PA1-641 was used in immunoprecipitation to characterize a new neuron-specific kinesin-like protein
|Saito N,Okada Y,Noda Y,Kinoshita Y,Kondo S,Hirokawa N||Neuron (18:425)||1997|
KIFC2; Kinesin family member C2; kinesin-like protein KIFC2