Invitrogen

LCMT1 Monoclonal Antibody (OTI 1G5)

Product Image Gallery

LCMT1 Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of LCMT1 in COS7 cells. Cells were transfected with a plasmid overexpressing LCMT1 and probed with a LCMT1 monoclonal antibody (Product # MA5-25598).

LCMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LCMT1 monoclonal antibody (Product # MA5-25598).

LCMT1 Antibody in Western Blot (WB) — Western blot analysis of LCMT1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with LCMT1 (Product # MA5-25598) monoclonal antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI 1G5

Immunogen

Full length human recombinant protein of LCMT1 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724585

Target Information

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: [phosphatase 2A protein]-leucine-carboxy methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1; CGI-68; LCMT; LCMT1; Leucine carboxyl methyltransferase 1; Phosphatase 2A protein-leucine-carboxy methyltransferase 1; PP2A methyltransferase; protein phosphatase methyltransferase 1; Protein-leucine O-methyltransferase

Gene Aliases: CGI-68; LCMT; LCMT1; PPMT1

UniProt ID: (Human) Q9UIC8

Entrez Gene ID: (Human) 51451

Molecular Function: methyltransferase transferase