Invitrogen

LCMT1 Monoclonal Antibody (OTI 1G5)

Product Image Gallery

LCMT1 Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of LCMT1 in COS7 cells. Cells were transfected with a plasmid overexpressing LCMT1 and probed with a LCMT1 monoclonal antibody (Product # MA5-25598).

LCMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LCMT1 monoclonal antibody (Product # MA5-25598).

LCMT1 Antibody in Western Blot (WB) — Western blot analysis of LCMT1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with LCMT1 (Product # MA5-25598) monoclonal antibody.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI 1G5

Immunogen

Full length human recombinant protein of LCMT1 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724585

Target Information

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [phosphatase 2A protein]-leucine-carboxy methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1; Leucine carboxyl methyltransferase 1; protein phosphatase methyltransferase 1; Protein-leucine O-methyltransferase

Gene Aliases: CGI-68; LCMT; LCMT1; PPMT1

UniProt ID: (Human) Q9UIC8

Entrez Gene ID: (Human) 51451

Molecular Function: methyltransferase transferase