Invitrogen

LCMT1 Monoclonal Antibody (OTI 1G5)

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Datasheet

Protocols

Questions & Answers

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LCMT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of LCMT1 in COS7 cells. Cells were transfected with a plasmid overexpressing LCMT1 and probed with a LCMT1 monoclonal antibody (Product # MA5-25598).

LCMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LCMT1 monoclonal antibody (Product # MA5-25598).

LCMT1 Antibody in Western Blot (WB) — Western blot analysis of LCMT1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with LCMT1 (Product # MA5-25598) monoclonal antibody.

Product Details

MA5-25598

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	OTI 1G5
Immunogen	Full length human recombinant protein of LCMT1 produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.62 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724585

Target Information

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [phosphatase 2A protein]-leucine-carboxy methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1; Leucine carboxyl methyltransferase 1; protein phosphatase methyltransferase 1; Protein-leucine O-methyltransferase

Gene Aliases: CGI-68; LCMT; LCMT1; PPMT1

UniProt ID: (Human) Q9UIC8

Entrez Gene ID: (Human) 51451

Molecular Function: methyltransferase