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Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:4,000 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
1H6C11 |
Immunogen |
LIG4 Fusion Protein Ag3385 (606-911 aa encoded by BC037491) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: ASGKLASKH LYIGGDDEPQ EKKRKAAPKM KKVIGIIEHL KAPNLTNVNK ISNIFEDVEF CVMSGTDSQP KPDLENRIAE FGGYIVQNPG PDTYCVIAGS ENIRVKNIIL SNKHDVVKPA WLLECFKTKS FVPWQPRFMI HMCPSTKEHF AREYDCYGDS YFIDTDLNQL KEVFSGIKNS NEQTPEEMAS LIADLEYRYS WDCSPLSMFR RHTVYLDSYA VINDLSTKNE GTRLAIKALE LRFHGAKVVS CLAEGVSHVI IGEDHSRVAD FKAFRRTFKR KFKILKESWV TDSIDKCELQ EENQYLI (606-911 aa encoded by BC037491 )
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
Gene Aliases: LIG4; LIG4S
UniProt ID: (Human) P49917
Entrez Gene ID: (Human) 3981
Molecular Function:
DNA metabolism protein
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