Fig.1) Western blot analysis was performed on nuclear enriched extracts (30 µglysate) of MCF7 (Lane 1), HeLa ( Lane 2), Hep G2 (Lane 3), Jurkat (Lane 4) and HEK-293 (Lane 5). Fig2.) Likewise, western blot analysis was performed on tissue extracts ( 30 µglysate) of Mouse Liver, Mouse Spleen and Mouse Lung. The blots were probed with LXR alpha/beta Rabbit polyclonal Antibody (Product# PA1-331B, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/ml, 1:2500 dilution). A 40 kDa band corresponding to LXR beta was observed across the cell lines tested. Apart from this in Fig. 2, 50 kDa corresponding to LXR alpha form is also observed across all tissue extracts tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C L(432) R L Q D K K L P P L L S E(445) of human LXR alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-331B detects recombinant human liver X receptor (LXR).
PA1-331B has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~64 kDa and ~80 kDa protein representing recombinant human LXR alpha and beta.
The PA1-331B immunogen is a synthetic peptide corresponding to residues C L(432) R L Q D K K L P P L L S E(445) of human LXR alpha. This sequence also corresponds to amino acids 442-455 of the human LXR beta. PA1-331B immunizing peptide (Cat. # PEP-115) is available for use in neutralization and control experiments.
Liver X receptors (LXRs), also known as RLD1, are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.
The corresponding gene for the LXR is NR1H3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.