Immunofluorescence analysis of Laminin was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Laminin Rabbit Polyclonal Antibody (PA516287) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing extracellular matrix localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Pig, Zebrafish, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Laminin isolated from a rat yolk sac tumor cell line|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 10 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
PA5-16287 targets Laminin in IHC (P) applications and shows reactivity with Human and Rat samples.
The PA5-16287 immunogen is laminin isolated from a rat yolk sac tumor cell line.
Extracellular matrix (ECM) surrounds and separates most tissue compartments, determines tissue architecture and dynamically interacts with its surrounding cells. The basement membrane is a part of the ECM and is composed of collagens, proteoglycans, elastin and several glycoproteins of which laminin is the most abundant. Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains Dystroglycan is a major laminin receptor in muscle and epithelia, and nidogen is a high-affinity laminin-binding protein important for basement-membrane assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration.
PA5-16287 was used in immunohistochemistry - frozen section to analyze extracellular matrix protein expression during caudal fin regeneration in zebrafish.
|Govindan J,Iovine MK||Gene expression patterns : GEP (19:21)||2015|
Masticatory muscles of mouse do not undergo atrophy in space.
PA5-16287 was used in immunohistochemistry - free floating to study the effect of gravity on masticatory and appendicular muscles in the mouse
|Philippou A,Minozzo FC,Spinazzola JM,Smith LR,Lei H,Rassier DE,Barton ER||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:2769)||2015|
Two waves of anisotropic growth generate enlarged follicles in the spiny mouse.
PA5-16287 was used in immunohistochemistry - paraffin section to study the development of skin appendages in the spiny mouse with the help of developmental biology and numerical modeling approaches
|Montandon SA,Tzika AC,Martins AF,Chopard B,Milinkovitch MC||EvoDevo (5:null)||2015|
Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
PA5-16287 was used in immunohistochemistry to report that Tsp4b is essential for muscle attachment and extracellular matrix assembly at myotendinous junctions
|Subramanian A,Schilling TF||eLife (3:null)||2014|
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
PA5-16287 was used in immunohistochemistry to study the ability of viral expression of the EA and EB peptides of IGF-1 in rat skeletal muscle to increase muscle mass
|Brisson BK,Spinazzola J,Park S,Barton ER||American journal of physiology. Endocrinology and metabolism (306:E965)||2014|
Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland.
PA5-16287 was used in immunohistochemistry to determine how label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region create stem cells in the sweat gland
|Leung Y,Kandyba E,Chen YB,Ruffins S,Kobielak K||PloS one (8:null)||2013|
Rescue of dystrophic skeletal muscle by PGC-1¿ involves restored expression of dystrophin-associated protein complex components and satellite cell signaling.
PA5-16287 was used in immunohistochemistry to study the mechanisms underlying the beneficial effects of AAV6-mediated gene transfer of PGC-1alpha on dystrophic skeletal muscle using a murine model
|Hollinger K,Gardan-Salmon D,Santana C,Rice D,Snella E,Selsby JT||American journal of physiology. Regulatory, integrative and comparative physiology (305:R13)||2013|
Phenotypic characteristics and proliferative activity of hyperplastic ductule cells in cholangiofibrosis induced by thioacetamide in rats.
PA5-16287 was used in immunohistochemistry to study the expression of cholangiocyte and hepatocyte markers by hyperplastic ductule cells in a rat model of cholangiofibrosis
|Hata M,Iida H,Yamanegi K,Yamada N,Ohyama H,Hirano H,Nakasho K,Terada N||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (65:351)||2013|
Hindlimb muscle morphology and function in a new atrophy model combining spinal cord injury and cast immobilization.
PA5-16287 was used in immunohistochemistry to characterize a novel atrophy model that utilizes a combination of spinal cord contusion injury and cast immobilization
|Ye F,Baligand C,Keener JE,Vohra R,Lim W,Ruhella A,Bose P,Daniels M,Walter GA,Thompson F,Vandenborne K||Journal of neurotrauma (30:227)||2013|
Regenerative responses in slow- and fast-twitch muscles following moderate contusion spinal cord injury and locomotor training.
PA5-16287 was used in immunohistochemistry to study the effects of treadmill training on the recovery of slow- and fast twitch muscles in a rat model following moderate spinal cord contusion injury
|Jayaraman A,Liu M,Ye F,Walter GA,Vandenborne K||European journal of applied physiology (113:191)||2013|
The nanomechanical signature of breast cancer.
PA5-16287 was used in immunohistochemistry to study the nano-mechanical profiles of normal and malignant breast tissue
|Plodinec M,Loparic M,Monnier CA,Obermann EC,Zanetti-Dallenbach R,Oertle P,Hyotyla JT,Aebi U,Bentires-Alj M,Lim RY,Schoenenberger CA||Nature nanotechnology (7:757)||2012|
The effect of valproic acid and oxcarbazepine on the distribution of adhesion molecules in embryo implantation.
PA5-16287 was used in immunohistochemistry to study the effect of valproic acid and oxcarbazepine on the distribution of adhesion molecules in embryo implantation
|Gürgen SG,Erdo¿an D,Co¿kun ZK,Cansu A||Toxicology (292:71)||2012|
Topographic characterization and protein quantification of esophageal basement membrane for scaffold design reference in tissue engineering.
PA5-16287 was used in immunohistochemistry to study the topography and protein content of esophageal basement membrane to aid the design of scaffolds for tissue engineering
|Li Y,Zhu Y,Yu H,Chen L,Liu Y||Journal of biomedical materials research. Part B, Applied biomaterials (100:265)||2012|
Immunocytochemistry to study myogenesis in zebrafish.
PA5-16287 was used in immunocytochemistry to report on immunocytochemical methods suitable for the study of zebrafish myogenesis
|Bird NC,Windner SE,Devoto SH||Methods in molecular biology (Clifton, N.J.) (798:153)||2011|