Immunofluorescent analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Laminin Receptor monoclonal antibody (Product # MA5-12840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgM|
|Immunogen||Small cell lung carcinoma N592 live cells|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/mL|
|Western Blot (WB)||1:10-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12840 targets Laminin Receptor in FACS, WB, ICC/IF, and IHC (P) applications and shows reactivity with Human samples.
The MA5-12840 immunogen is small cell lung carcinoma N592 live cells.
Laminin, one of the major glycoproteins of basement membrane, displays multiple biological activities which are mediated through its interaction with specific cell membrane receptors. Overexpression of laminin receptor is reportedly an independent prognostic factor in breast cancer and correlates with the dissemination of the tumor cells in bone marrow. Laminin receptor appears to play an important role in tumor invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The effects of cytotoxic necrotizing factor 1 expression in the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice.
MA5-12840 was used in western blot to study the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice and the effects of cytotoxic necrotizing factor 1 expression
|Chang AC,Krishnan S,Prasadarao NV||Virulence (7:806)||2016|
Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence.
MA5-12840 was used in western blot to study the role of lowered lung cellular senescence in the mechanism by which enteric rapamycin reduces the susceptibility of aged mice to Streptococcus pneumoniae infection
|Hinojosa CA,Mgbemena V,Van Roekel S,Austad SN,Miller RA,Bose S,Orihuela CJ||Experimental gerontology (47:958)||2012|
Green tea polyphenol EGCG sensing motif on the 67-kDa laminin receptor.
MA5-12840 was used in western blot to study the functional domain of 67kDa laminin responsible for the anti-cancer activity of the green tea polyphenol (-)-epigallocatechin-3-O-gallate
|Fujimura Y,Sumida M,Sugihara K,Tsukamoto S,Yamada K,Tachibana H||PloS one (7:null)||2012|
Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-¿-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells.
MA5-12840 was used in blocking or activating experiment to study the roles of NFkB and the 67-kD laminin receptor in the mechanism by which a green tea polyphenol inhibits TNF-alpha-induced MCP-1 production by HUVECs
|Wang ZM,Gao W,Wang H,Zhao D,Nie ZL,Shi JQ,Zhao S,Lu X,Wang LS,Yang ZJ||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (33:1349)||2014|
Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor.
MA5-12840 was used in blocking or activating experiment to study the molecular mechanism for the inhibition of peptidoglycan-dependent TLR2 signalling by a Green tea polyphenol.
|Byun EH,Omura T,Yamada K,Tachibana H||FEBS letters (585:814)||2011|
Human T cells express a functional ionotropic glutamate receptor GluR3, and glutamate by itself triggers integrin-mediated adhesion to laminin and fibronectin and chemotactic migration.
MA5-12840 was used in blocking or activating experiment to study the role of GluR3 in the glutamate triggered adhesion and chemotactic migration of human T cells
|Ganor Y,Besser M,Ben-Zakay N,Unger T,Levite M||Journal of immunology (Baltimore, Md. : 1950) (170:4362)||2003|
High expression of 67-kDa laminin receptor relates to the proliferation of leukemia cells and increases expression of GM-CSF receptor.
MA5-12840 was used in flow cytometry to study the role of high level expression of the 67-kDa laminin receptor in GM-CSF receptor expression and proliferation of leukemia cells
|Ando K,Miyazaki Y,Sawayama Y,Tominaga S,Matsuo E,Yamasaki R,Inoue Y,Iwanaga M,Imanishi D,Tsushima H,Fukushima T,Imaizumi Y,Taguchi J,Yoshida S,Hata T,Tomonaga M||Experimental hematology (39:179)||2011|
Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.
MA5-12840 was used in immunohistochemistry to investigate the prognostic value of the 67-kd laminin receptor in cervical cancer and its relationship with high-risk HPV types
|Branca M,Giorgi C,Ciotti M,Santini D,Di Bonito L,Costa S,Benedetto A,Bonifacio D,Di Bonito P,Paba P,Accardi L,Mariani L,Syrjänen S,Favalli C,Syrjänen K||Acta cytologica (50:6)||2006|
Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone.
MA5-12840 was used in immunocytochemistry to study the role of laminin on allergic airway remodelling in chronic allergic lung inflammation.
|Christie PE,Jonas M,Tsai CH,Chi EY,Henderson WR||The European respiratory journal (24:107)||2004|