|Tested species reactivity||Bacteria|
|Published species reactivity||Bacteria|
|Host / Isotype||Goat / IgG|
|Immunogen||Whole cell prep of Lipid A from E. coli O157|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-73178 is crossreactive with numerous members of the Enterobacteriaciae.
PA1-73178 has been successfully used in immunofluorescence, immunocytochemistry, western blot, immunohistochemistry and ELISA applications.
The PA1-73178 antibody was raised against purified Lipid A Lipopolysaccharide.
Store at 2-8°C up to 6 months. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Purification and characterization of lipopolysaccharides from six strains of non-O157 Shiga toxin-producing Escherichia coli.
PA1-73178 was used in western blot to isolate LPS and O-antigen from six Shiga toxin-producing Escherichia coli strains and assess the ability of commercial antibodies to recognize them
|Stromberg LR,Stromberg ZR,Banisadr A,Graves SW,Moxley RA,Mukundan H||Journal of microbiological methods (116:1)||2015|