|Flow Cytometry (Flow)||0.25 µg/test|
|Flow Cytometry (Flow)||See 3 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Syrian hamster / IgG|
|Recommended Isotype Control||Syrian Hamster IgG Isotype Control, FITC, eBioscience™|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.
Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide -receptive H-2Kb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5E6; cytomegalovirus resistance 1; Killer cell lectin-like receptor 3; Killer cell lectin-like receptor 6; Killer cell lectin-like receptor 8; Ly-49c; Ly-49f; Ly-49h; Lymphocyte antigen 49c; Lymphocyte antigen 49f; Lymphocyte antigen 49h; natural killer cell-associated antigen 2; Nk2.1; T-cell surface glycoprotein Ly-49C; T-cell surface glycoprotein Ly-49F; T-cell surface glycoprotein Ly-49H
Gene Aliases: 5E6; Cmv-1; Cmv1; Klra3; Klra6; Klra8; Ly-49c; Ly-49f; Ly-49h; Ly49-f; Ly49-h; Ly49C; Ly49F; Ly49H; Ly49u<129>; Nk-2; NK-2.1; Nk2; Nk2.1
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