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Immunofluorescent analysis of CD115 (green) showing staining in the cytoplasm and membrane of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD115 monoclonal antibody (Product # RT601PABX) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||v-ras transformed rat NRK cells expressing transduced human CSF-1R|
|Storage buffer||PBS, pH 7.4|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RT601PABX targets CD115 in ICC/IF, IHC, and Neu applications and shows reactivity with Human samples.
The RT601PABX immunogen is v-ras transformed rat NRK cells expressing transduced human CSF-1R.
RT601PABX detects CD115 which has a predicted molecular weight of approximately 106 kDa.
The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C-FMS; CD115; CD115 antigen; colony stimulating factor 1 receptor; CSF-1 receptor; CSF1R; CSFR; FIM2; FMS; FMS proto-oncogene; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; McDonough feline sarcoma viral v-fms oncogene homolog; proto-oncogene c-Fms
C-FMS; CD115; CSF-1R; CSFR; FIM2; FMS; HDLS; M-CSF-R
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Immunity and defense Stress response Oncogenesis Developmental processes Gametogenesis Embryogenesis Cell structure and motility Cell motility Cell proliferation and differentiation