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Recommended positive controls: LS174T cells and skin.
Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For staining formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, for 10 min at 37ºC. Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
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