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GKN1 Antibody (MA5-24367) in IHC (F)
Immunohistochemical analysis of GKN1 in perfusion fixed frozen sections of mouse intestine. Samples were incubated with GKN1 monoclonal antibody (Product # MA5-24367) using a dilution of 25 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to gastric glands.
Product Details
MA5-24367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
759821
Immunogen
E. coli-derived recombinant mouse Gastrokine 1 Tyr38-Tyr201
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605592
Product Specific Information
This antibody detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Gastrokine 1, also known as GKN1 and CA11, is a 185 amino acid containing secreted protein belonging to the gastrokine family with a BRICHOS domain and an asn-rich region. GKN1 (also known as an antioncogene) is reported to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. Differential display of normal and cancerous gastric tissue suggested that this protein is predominantly expressed in stomach and the expression is down-regulated to considerable frequency in cancer tissue or gastric cancer cell lines examined. This extra cellular protein is co-secreted with mucins and is a signaling growth factor with mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa antrum mucosa protein; AMP-18; foveolin; Gastrokine-1; Protein CA11 homolog
Gene Aliases: 2200002K21Rik; AMP-18; Amp18; BRICD1; Ca11; Fov; Gkn1
UniProt ID: (Mouse) Q9CR36
Entrez Gene ID: (Mouse) 66283
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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