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Invitrogen
MDM2 Monoclonal Antibody (2A10)
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FIGURE: 1 / 2
MDM2 Antibody (MA1-25896) in WB
MDM2 Monoclonal Antibody (2A10) detects MDM2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µgRaji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.7.5 % SDS-PAGE. MDM2 Monoclonal Antibody (2A10) (Product # MA1-25896) dilution: 1:500.
Product Details
MA1-25896
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2A10
Immunogen
Human MDM2 protein.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
50 µg
Storage conditions
4°C, do not freeze
Shipping conditions
Wet ice
RRID
Product Specific Information
Recommended positive controls: A549 , H1299.
Heat pretreatment is required for paraffin sections.
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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