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Immunofluorescent analysis of MAP2K1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing MAP2K1 (Product# MA1-095), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
|Tested species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Protein expressed in 293T cell transfected with human MAP2K1 expression vector|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5-20µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10,000-1:100,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MEKK1, MKK1, MAPKK1, Mek1, PRKMK1, MEK1, Prkmk1, CFC3
ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, dual specificity mitogen-activated protein kinase kinase 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase 1, mitogen activated protein kinase kinase 1, protein kinase, mitogen activated, kinase 1, p45, MAPKK1, MEK1, MKK1, PRKMK1