|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant HLA-E refolded with beta-2 Microglobulin and peptide.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
This antibody reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7) but also may exhibits much broader cross-reactivity with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
A suggested positive control for this product is spleen tissue.
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies.
MA1-19356 was used in ELISA to study the cross-reaction of some of the natural HHLA-E antibodies with HLA-Ia alleles
|Ravindranath MH,Taniguchi M,Chen CW,Ozawa M,Kaneku H,El-Awar N,Cai J,Terasaki PI||Molecular immunology (47:1121)||2010|