Western blot analysis of MAP2K3 was performed with 10 µg of A549 cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against MAP2K3 (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (NP0322BOX), XCell SureLock™ Electrophoresis System (EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (37539) for 1 hour at room temperature. MAP2K3 was detected at ~ 40 kDa using MAP2K3 Rabbit polyclonal antibody (Product # PA5-17480) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Rabbit (product # 31461) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to GAPDH (36 kDa). MAP2K3 Antibody (Product # PA5-17480) confirms silencing of MAP2K3 expression.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal sequence of human MKK3|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with MEK1, MEK2, MKK4 or MKK6.
dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPK/ERK kinase 3; MAPKK 3; MEK 3; mitogen activated protein kinase kinase 3; protein kinase, mitogen-activated, kinase 3; SAPK kinase 2; stress-activated protein kinase kinase 2
AW212142; MAP2K3; MAPKK3; MEK3; MKK3; mMKK3b; PRKMK3; SAPKK-2; SAPKK2; SKK2