Immunofluorescent analysis of MKLP1 showing staining in the midbody of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a MKLP1 polyclonal antibody (Product # PA5-31773) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 214 of Human MKLP1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31773 targets MKLP1 in WB applications and shows reactivity with Human samples.
The PA5-31773 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 214 of Human MKLP1.
The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms.
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