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Immunofluorescent analysis of MKLP1 showing staining in the midbody of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a MKLP1 polyclonal antibody (Product # PA5-31773) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 214 of Human MKLP1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31773 targets MKLP1 in WB applications and shows reactivity with Human samples.
The PA5-31773 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 214 of Human MKLP1.
The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CHO1; kinesin-like 5 (mitotic kinesin-like protein 1); kinesin-like protein 5; kinesin-like protein KIF23; KNSL5; mitotic kinesin-like 1; mitotic kinesin-like protein 1; MKLP-1; MKLP1
CHO1; KIF23; KNSL5; MKLP-1; MKLP1