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MM600CB targets IL-6 in ELISA, IHC (P), IP, Neutralization and WB applications and shows reactivity with mouse samples.
The MM600CB immunogen is recombinant mouse IL-6.
MM600CB detects IL-6 which has a predicted molecular weight of approximately 22 kDa.
The MM600CB mouse IL6 antibody (biotinylated conjugate of clone 20F3) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM600D (clone 32C11). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody MM600CB (clone 20F3) and antibody MM600D (clone 32C11) have successfully been used in combination with recombinant mouse IL6 protein SMIL6 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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